变形的分子印迹纳米凝胶可提高等离子体传感的灵敏度。
Deformable molecularly imprinted nanogels permit sensitivity-gain in plasmonic sensing.
机构信息
University of Campania Luigi Vanvitelli, Department of Engineering, Via Roma 29, 81031, Aversa, Italy.
University of Trento, Department of Industrial Engineering, BIOtech Research Center, Via Delle Regole 101, Mattarello, 38123, Trento, Italy.
出版信息
Biosens Bioelectron. 2020 May 15;156:112126. doi: 10.1016/j.bios.2020.112126. Epub 2020 Feb 28.
Soft molecularly imprinted nanogels (nanoMIPs), selective for human transferrin (HTR), were prepared via a template assisted synthesis. Owing to their soft matter, the nanoMIPs were observed to deform at binding to HTR: while no relevant changes were observed in the hydrodynamic sizes of HTR-free compared to HTR-loaded nanoMIPs, the HTR binding resulted in a significant increment of the nanoMIP stiffness, with the mean Young's modulus measured by AFM passing from 17 ± 6 kPa to 56 ± 18 kPa. When coupled to a plastic optical fibre (POF) plasmonic platform, the analyte-induced nanoMIP-deformations amplified the resonance shift, enabling to attain ultra-low sensitivities (LOD = 1.2 fM; linear dynamic range of concentrations from 1.2 fM to 1.8 pM). Therefore, soft molecularly imprinted nanogels that obey to analyte-induced deformation stand as a novel class of sensitivity-gain structures for plasmonic sensing.
通过模板辅助合成，制备了对人转铁蛋白（HTR）具有选择性的软质分子印迹纳米凝胶（nanoMIPs）。由于其软物质特性，在与 HTR 结合时，nanoMIPs 被观察到发生变形：与未结合 HTR 的 nanoMIPs 相比，结合 HTR 后 nanoMIPs 的流体力学尺寸没有变化，但 HTR 结合导致 nanoMIPs 硬度显著增加，通过 AFM 测量的平均杨氏模量从 17 ± 6 kPa 增加到 56 ± 18 kPa。当与塑料光纤（POF）等离子体平台耦合时，分析物诱导的 nanoMIP 变形放大了共振位移，从而实现了超低的灵敏度（LOD=1.2 fM；浓度的线性动态范围从 1.2 fM 到 1.8 pM）。因此，遵循分析物诱导变形的软质分子印迹纳米凝胶是等离子体传感的一类新型灵敏度增益结构。
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