胎盘内皮素转换酶-1 在子痫前期减少。
Placental endothelin-converting enzyme-1 is decreased in preeclampsia.
机构信息
Medical College of Wisconsin, Dept. Of Obstetrics and Gynecology, 9200 West Wisconsin Ave, Milwaukee, WI 53226, United States.
Medical College of Wisconsin, Dept. Of Obstetrics and Gynecology, 9200 West Wisconsin Ave, Milwaukee, WI 53226, United States.
出版信息
Pregnancy Hypertens. 2020 Apr;20:108-110. doi: 10.1016/j.preghy.2020.04.001. Epub 2020 Apr 6.
Endothelin-converting enzyme-1(ECE-1) is a key regulatory enzyme in the processing of endothelin-1 (ET-1). We quantified and localized ECE-1 in normal and preeclamptic placentas. Normal (n=6) and preeclamptic (n=6) placentas were serially sectioned for immunofluorescence (IF). Cell type specific markers identified endothelial, trophoblast, macrophage, smooth muscle, and fibroblast cells. Quantitative analyses were performed by western blot and ELISA. IF identified ECE-1 expression within the stroma and villous space. Cellular localization of ECE-1 was limited to endothelial membranes. There was significantly less ECE-1 in preeclamptic placentas, suggesting ECE-1 is important for proper regulation of ET-1 within the placenta.
内皮素转换酶 1（ECE-1）是内皮素-1（ET-1）加工过程中的关键调节酶。我们对正常和子痫前期胎盘中的 ECE-1 进行了定量和定位。对正常（n=6）和子痫前期（n=6）胎盘进行连续切片进行免疫荧光（IF）。细胞类型特异性标志物鉴定了内皮细胞、滋养层细胞、巨噬细胞、平滑肌细胞和成纤维细胞。通过 Western blot 和 ELISA 进行定量分析。IF 鉴定了 ECE-1 在基质和绒毛腔内的表达。ECE-1 的细胞定位仅限于内皮膜。子痫前期胎盘中的 ECE-1 明显减少，表明 ECE-1 对于胎盘内 ET-1 的正常调节很重要。
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