Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran.

BACKGROUND

is an important cause of healthcare-associated infection. Carbapenemases have increasingly been reported in Enterobacteriaceae, especially in .

PROPOSE

The objective of this study was to determine antibiotic resistance patterns, and the molecular epidemiology of multidrug resistant isolates, obtained from hospitalized patients in Shiraz, Iran.

METHODS

In this study, 60 isolates were collected from Nemazee and Faghihi referral hospitals. Antibiotic susceptibility testing and MIC were performed by disk diffusion test and Epsilometer (E)-test strips, respectively. Carbapenemase genes were identified by polymerase chain reaction and sequencing. Then, clonal relationships were analyzed, using PFGE.

RESULTS

Thirty-three out of 60 isolates were resistant to carbapenems. Among the isolates, 86.6% were multidrug resistant (MDR). Polymyxin B (18.3%) and tigecycline (23.3%) were shown to be the most active agents against isolates. In our study, the high prevalence of (45%) and (10%) was detected.

CONCLUSION

The results of this study revealed the widespread carbapenemase gene between different wards in hospitals as a risk factor for treatment options. PFGE analysis showed 11 clusters and 3 singletons based on an 80% similarity level. Also, PFGE analysis showed that there were similar genetic patterns among isolates and these patterns were responsible for the distribution of infection in hospitals.