Planta LLC, Moscow, Russia.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Nat Biotechnol. 2020 Aug;38(8):944-946. doi: 10.1038/s41587-020-0500-9. Epub 2020 Apr 27.
Autoluminescent plants engineered to express a bacterial bioluminescence gene cluster in plastids have not been widely adopted because of low light output. We engineered tobacco plants with a fungal bioluminescence system that converts caffeic acid (present in all plants) into luciferin and report self-sustained luminescence that is visible to the naked eye. Our findings could underpin development of a suite of imaging tools for plants.
具有自主发光能力的植物,通过在质体中表达细菌生物发光基因簇进行工程改造,但由于发光效率低,尚未得到广泛应用。我们利用真菌生物发光系统对烟草植物进行工程改造,该系统将咖啡酸(所有植物中都存在)转化为荧光素,并报告肉眼可见的自我持续发光。我们的研究结果为开发一系列植物成像工具奠定了基础。