巴西圣保罗州库巴唐市和桑托斯市焊工颊黏膜细胞的遗传毒性和细胞毒性效应评估。
Evaluation of genotoxic and cytotoxic effects in buccal mucosa cells of welders in the cities of Cubatão and Santos, state of São Paulo, Brazil.
作者信息
do Vale Laiane Damara Oliveira, da Silva Victor Hugo Pereira, de Almeida Flavio Rossi, Ribeiro Daniel Araki, da Silva Daniela Martins
机构信息
UNIP - Santos (SP), Brazil.
Laboratory of Toxicogenomics, UNIFESP - Santos (SP), Brazil.
出版信息
Rev Bras Med Trab. 2017 Dec 1;15(4):303-309. doi: 10.5327/Z1679443520170012. eCollection 2017.
BACKGROUND
Approximately 5 million workers are estimated to be occupationally exposed to welding fumes worldwide. Nickel and chromium are genotoxic metals found in welding fumes, therefore welders are exposed to these metals at the workplace.
OBJECTIVE
The objective of the present study was to investigate the frequency of cytotoxic and genetic damage in cells harvested from the oral mucosa of welders and also from a group of workers not exposed to metallic fumes.
METHODS
A total of 44 individuals, divided into 2 groups - welders and non-welders - were compared using the micronucleus assay technique and cell death (pyknosis, karyorrhexis and karyolysis) on buccal mucosa cells of welding workers. The examined cells were stained with Feulgen/Fast Green. Welders exhibited higher frequency (p<0.05) of cytotoxicity than the group of volunteers not exposed to metallic fumes.
CONCLUSION
The results of this preliminary study suggest that the frequency of cytotoxic damage in buccal mucosa cells might be higher among welders compared to non-welders.
背景
据估计，全球约有500万工人职业性接触焊接烟尘。镍和铬是焊接烟尘中发现的具有遗传毒性的金属，因此焊工在工作场所会接触到这些金属。
目的
本研究的目的是调查从焊工口腔黏膜以及一组未接触金属烟尘的工人采集的细胞中细胞毒性和遗传损伤的频率。
方法
总共44名个体分为两组——焊工组和非焊工组——使用微核试验技术以及对焊接工人颊黏膜细胞的细胞死亡（核固缩、核碎裂和核溶解）情况进行比较。检查的细胞用福尔根/固绿染色。焊工组的细胞毒性频率高于未接触金属烟尘的志愿者组（p<0.05）。
结论
这项初步研究的结果表明，与非焊工相比，焊工颊黏膜细胞中的细胞毒性损伤频率可能更高。
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