深部脑刺激通过增加突触可塑性改善脊髓损伤大鼠的运动功能。
Deep Brain Stimulation Improves Motor Function in Rats with Spinal Cord Injury by Increasing Synaptic Plasticity.
机构信息
Department of Orthopedics, Xuanwu Hospital Capital Medical University, Beijing, China; The fourth Department of Orthopedics, Jincheng General Hospital, Jincheng, Shanxi Province, China.
Beijing Key Laboratory of Mental Disorders & The National Clinical Research Center for Mental Disorder, Beijing Anding Hospital, Capital Medical University, Beijing, China; Department of Neurology, Jincheng General Hospital, Jincheng, Shanxi Province, China.
出版信息
World Neurosurg. 2020 Aug;140:e294-e303. doi: 10.1016/j.wneu.2020.05.029. Epub 2020 May 11.
OBJECTIVE
To investigate the effect of deep brain stimulation (DBS) on rats with spinal cord injury (SCI) and its possible molecular mechanism.
METHODS
A rat SCI model was prepared using a modified Allen method. The animals were randomly divided into 3 groups (n = 12 per group): the sham group, the SCI group, and the SCI + DBS group. Then, DBS was applied to the rats in the SCI + DBS group for half an hour per day for 4 weeks. Basso, Beattie, and Bresnahan scores were used to assess spinal function.
RESULTS
DBS significantly improved hindlimb motor function in SCI rats, and the protein expression levels of brain-derived neurotrophic factor, the mammalian target of rapamycin, tropomyosin-related kinase B, protein kinase B, p70 ribosomal S6 protein kinase, postsynaptic density protein 95, and synaptophysin increased correspondingly.
CONCLUSIONS
DBS improves motor function in rats with SCI by increasing synaptic plasticity via tropomyosin-related kinase B-protein kinase B-mammalian target of rapamycin pathway.
目的
探讨深部脑刺激（DBS）对脊髓损伤（SCI）大鼠的影响及其可能的分子机制。
方法
采用改良 Allen 法制备大鼠 SCI 模型。动物随机分为 3 组（每组 n=12）：假手术组、SCI 组和 SCI+DBS 组。然后，SCI+DBS 组大鼠每天接受 DBS 治疗半小时，持续 4 周。采用 Basso、Beattie 和 Bresnahan 评分评估脊髓功能。
结果
DBS 显著改善了 SCI 大鼠后肢运动功能，脑源性神经营养因子、雷帕霉素靶蛋白、原肌球蛋白相关激酶 B、蛋白激酶 B、p70 核糖体 S6 蛋白激酶、突触后密度蛋白 95 和突触小体素的蛋白表达水平相应增加。
结论
DBS 通过增加原肌球蛋白相关激酶 B-蛋白激酶 B-雷帕霉素靶蛋白通路的突触可塑性来改善 SCI 大鼠的运动功能。
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