Undabarrena A, Salvà-Serra F, Jaén-Luchoro D, Castro-Nallar E, Mendez K N, Valencia R, Ugalde J A, Moore E R B, Seeger M, Cámara B
Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Departamento de Química & Centro de Biotecnología Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María, Valparaíso, Chile.
Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden; Microbiology, Department of Biology, University of the Balearic Islands, Palma de Mallorca, Spain.
Mar Genomics. 2018 Jul;40:13-17. doi: 10.1016/j.margen.2018.01.004. Epub 2018 Feb 15.
Rhodococcus sp. H-CA8f was isolated from marine sediments obtained from the Comau fjord, located in Northern Chilean Patagonia. Whole-genome sequencing was achieved using PacBio RS II platform, comprising one closed, complete chromosome of 6,19 Mbp with a 62.45% G + C content. The chromosome harbours several metabolic pathways providing a wide catabolic potential, where the upper biphenyl route is described. Also, Rhodococcus sp. H-CA8f bears one linear mega-plasmid of 301 Kbp and 62.34% of G + C content, where genomic analyses demonstrated that it is constituted mostly by putative ORFs with unknown functions, representing a novel genetic feature. These genetic characteristics provide relevant insights regarding Chilean marine actinobacterial strains.
红球菌属菌株H-CA8f是从位于智利巴塔哥尼亚北部的科 Mau 峡湾采集的海洋沉积物中分离出来的。使用PacBio RS II平台完成了全基因组测序,该基因组包含一条619 Mbp的闭合完整染色体,G+C含量为62.45%。该染色体拥有多种代谢途径,具有广泛的分解代谢潜力,其中描述了上位联苯途径。此外,红球菌属菌株H-CA8f带有一个301 Kbp的线性大质粒,G+C含量为62.34%,基因组分析表明,它主要由功能未知的假定开放阅读框组成,这代表了一种新的遗传特征。这些遗传特征为智利海洋放线菌菌株提供了相关见解。