不可逆水胶体与银纳米颗粒及洗必泰混合后的抗菌活性比较研究。

A comparative study on the antimicrobial activity of irreversible hydrocolloid mixed with silver nanoparticles and chlorhexidine.

作者信息

Nia Azadeh Farhang, Ataei Mahdi, Zeighami Habib

机构信息

Department of Prosthodontics, Dental School, Zanjan University of Medical Sciences, Zanjan, Iran.

Pharmacutical Biotechnology Research Center, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

出版信息

Dent Res J (Isfahan). 2020 Mar 17;17(2):120-125. eCollection 2020 Mar-Apr.

PMID:32435434

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7224260/

Abstract

BACKGROUND

Impressions taken from patients have the potential of cross-transmission of infection among dentistry personnel. The present study aimed to compare the antimicrobial activity of chlorhexidine (CHX) and silver nanoparticles (AgNPs) combined with irreversible hydrocolloid.

MATERIALS AND METHODS

This experimental study examined the antimicrobial effects of irreversible hydrocolloid mixed with silver nanoparticles and chlorhexidine using four groups, namely CHX (0.2%) solution and mouthwash mixed with irreversible hydrocolloid Groups 1 and 2), AgNPs (0.1 and 0.2%) (Groups 3 and 4), and specimens mixed with distilled water as a control group (Group 5) on bacterial strains, namely, Staphylococcu saureu s,Enterococcu sfaecali s,Pseudomona saeruginos a,Escherichi acoli, andStaphylococcu sepidermidi s through disc diffusion method. There were three replications per bacterial species. As data were not normally distributed, Kruskal-Wallis test was used at a significance level of 0.05.

RESULTS

No antimicrobial activity was observed in the control groups. In , CHX mouthwash had the highest antimicrobial activity, and AgNPs 0.1% and 0.2% groups had lower antimicrobial activity, and there was a significant difference between the two concentrations of AgNPs ( < 0.05). InE.faecali s, the effects of CHX compounds and AgNPs 0.2% were similar to each other and were higher than the effect of AgNPs 0.1% ( < 0.05). In , CHX compounds exhibited the highest efficacy relative to other materials ( < 0.05), and the AgNPs had no effect. In , AgNPs showed the highest growth inhibition zone, which had a significant difference compared to other materials ( ≤ 0.01), whereas the CHX compounds were not effective. InS.epidermidi s, the effect of CHX compounds was similar to one another and was higher than the effect of AgNPs ( ≤ 0.01).

CONCLUSION

According to our observations, the antimicrobial activity of AgNPs at 0.1 and 0.2% against five tested bacterial strains was similar to those of pure CHX 0.2% solution and CHX 0.2% mouthwash.

摘要

背景

从患者身上获取的印模有在牙科医护人员中交叉传播感染的可能性。本研究旨在比较洗必泰（CHX）和银纳米颗粒（AgNPs）与不可逆水胶体联合使用时的抗菌活性。

材料与方法

本实验研究使用四组来检测与银纳米颗粒和洗必泰混合的不可逆水胶体的抗菌效果，即CHX（0.2%）溶液和漱口水与不可逆水胶体混合（第1组和第2组）、AgNPs（0.1%和0.2%）（第3组和第4组），以及与蒸馏水混合的标本作为对照组（第5组），通过纸片扩散法检测对金黄色葡萄球菌、粪肠球菌、铜绿假单胞菌、大肠杆菌和表皮葡萄球菌等菌株的抗菌效果。每种细菌有三次重复。由于数据呈非正态分布，采用Kruskal-Wallis检验，显著性水平为0.05。

结果

对照组未观察到抗菌活性。在金黄色葡萄球菌中，CHX漱口水的抗菌活性最高，0.1%和0.2%的AgNPs组抗菌活性较低，两种浓度的AgNPs之间存在显著差异（P<0.05）。在粪肠球菌中，CHX化合物和0.2%的AgNPs的效果彼此相似，且高于0.1%的AgNPs的效果（P<0.05）。在铜绿假单胞菌中，CHX化合物相对于其他材料表现出最高的疗效（P<0.05），而AgNPs无效。在大肠杆菌中，AgNPs显示出最高的生长抑制圈，与其他材料相比有显著差异（P≤0.01），而CHX化合物无效。在表皮葡萄球菌中，CHX化合物的效果彼此相似，且高于AgNPs的效果（P≤0.01）。