采用匹配 Fenton 预氧化快速刺激生物修复土壤中粗原油。
Fast-stimulating bioremediation of macro crude oil in soils using matching Fenton pre-oxidation.
机构信息
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, 710055, Shaanxi, Xi'an, China; Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, China; Key Laboratory of Environmental Engineering, Shaanxi Province, China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, 710055, Shaanxi, Xi'an, China; Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, China; Key Laboratory of Environmental Engineering, Shaanxi Province, China.
出版信息
Chemosphere. 2020 Aug;252:126622. doi: 10.1016/j.chemosphere.2020.126622. Epub 2020 Apr 1.
This study aims at exploring the mechanism of fast-stimulating bioremediation of macro crude oil using matching Fenton pre-oxidation. The 80-day biodegradation experiment for soil S1 and S2, containing macro crude oil: C-C and C-C respectively, was conducted after Fenton pre-oxidation with three concentrations of HO (225 mM, 450 mM, and 900 mM). Experimental results indicated that the bioremediation efficiency of macro crude oil was up to 57.1% (8853 mg/kg, S1) and 64.4% (11,719 mg/kg, S2) for 80-day fast-stimulating bioremediation using matching Fenton pre-oxidation (450 mM HO), which was 1.8-2.6 times that (S1: 22.2-37.1%; S2: 36.1-39.6%) for slow-stimulating bioremediation using un-matching Fenton pre-oxidation. Furthermore, the high-throughput analysis revealed that genera Sedimentibacter, Caenispirillum, and Brevundimonas became the dominant bacteria after matching Fenton pre-oxidation. Meanwhile, the highest logarithmic growth rate of indigenous hydrocarbon degraders (IHD) was obtained (S1: 64% and S2: 60%) for fast-stimulating bioremediation. And the consumption of NH-N was up to 90% and 94% in S1 and S2 within 60 days for fast-stimulating bioremediation, approximately 1.4 and 2.2 times that (S1: 65% and 62%; S2: 47% and 41%) for slow-stimulating remediation. The results showed that the macro crude oil became the main carbon source for IHD for the fast-stimulating bioremediation, resulting in the rapid growth of IHD. Thus, this study provides a fast and efficient remediation technology for bioremediation of macro crude oil-contaminated soils.
本研究旨在探索匹配 Fenton 预氧化快速刺激生物修复大体积原油的机制。在进行 Fenton 预氧化后，对含有大体积原油（C-C 和 C-C 分别为 S1 和 S2）的土壤 S1 和 S2 进行了 80 天的生物降解实验，HO 的浓度分别为 225mM、450mM 和 900mM。实验结果表明，使用匹配的 Fenton 预氧化（450mM HO）进行 80 天的快速刺激生物修复，大体积原油的生物修复效率达到 57.1%（8853mg/kg，S1）和 64.4%（11719mg/kg，S2），是使用不匹配的 Fenton 预氧化进行缓慢刺激生物修复的 1.8-2.6 倍（S1：22.2-37.1%；S2：36.1-39.6%）。此外，高通量分析表明，Sedimentibacter、Caenispirillum 和 Brevundimonas 等属成为匹配 Fenton 预氧化后的优势细菌。同时，在快速刺激生物修复中获得了最高的土著烃降解菌（IHD）对数增长率（S1：64%和 S2：60%）。在快速刺激生物修复中，S1 和 S2 中 NH-N 的消耗达到 90%和 94%，在 60 天内分别为缓慢刺激修复的 1.4 和 2.2 倍（S1：65%和 62%；S2：47%和 41%）。结果表明，大体积原油成为 IHD 的主要碳源，导致 IHD 的快速生长。因此，本研究为大体积原油污染土壤的生物修复提供了一种快速有效的修复技术。
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