School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen AB10 7GJ, UK.
School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen AB10 7GJ, UK.
Sci Total Environ. 2020 Sep 1;733:139346. doi: 10.1016/j.scitotenv.2020.139346. Epub 2020 May 12.
The consumption of contaminated shellfish with marine toxins causes adverse socioeconomical, environmental and health impacts. The marine toxin okadaic acid (OA) provokes diarrhetic shellfish poisoning (DSP) syndrome characterized by severe gastrointestinal symptoms. Therefore, there is increasing interest in removing these toxins from the marine environment to protect shellfish harvesting sites. Photocatalysis is proposed as an efficient method to detoxify the marine environment. In this study, Prorocentrum lima was used to produce high purity DSP toxins, in particular OA, for degradation studies. The profiling, characterization and quantification of DSP toxins in the culture of P. lima were achieved by ultrahigh performance liquid chromatography coupled to quadrupole-time of flight mass spectrometry (UPLC-QTOF-MS) for accurate-mass full spectrum acquisition data. The effectiveness of UV/TiO system to degrade OA in seawater was assessed in lab-scale experiments and identification of transformation products was proposed based on the data obtained during analysis by UPLC-QTOF-MS. The detoxification potential of the UV/TiO system was investigated using the phosphatase inhibition assay. Sufficient amount of high-purity OA (25 mg, >90% purity) was produced in-house for use in photocatalysis experiments by simple reversed-phase flash chromatography. Complete degradation of OA was observed in seawater after 30 min and 7.5 min in deionized water. The rate constants fitted with the pseudo-first order kinetic model (R > 0.96). High-resolution mass spectrometry analysis of the photocatalyzed OA allowed tentative identification of four transformation products. Detoxification was achieved in parallel with the degradation of OA in deionized water and artificial ocean water (≤20 min) but not for seawater. Overall, results suggest that UV/TiO photocatalysis can be an effective approach for degrading OA and their TPs in the marine environment. To the best of our knowledge, this is the first report on the use of photocatalysis to degrade marine toxins and its promising potential to protect shellfish harvesting sites.
食用受污染的贝类和含有海洋毒素的贝类会对社会经济、环境和健康造成不良影响。海洋毒素 okadaic acid（OA）会引发腹泻性贝类中毒（DSP）综合征，其特征是严重的胃肠道症状。因此，人们越来越有兴趣从海洋环境中去除这些毒素，以保护贝类捕捞区。光催化被认为是一种有效的解毒海洋环境的方法。在这项研究中，Prorocentrum lima 被用于生产高纯度的 DSP 毒素，特别是 OA，用于降解研究。采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-QTOF-MS）对 P. lima 培养物中的 DSP 毒素进行了分析，实现了对 DSP 毒素的分析、鉴定和定量，采用精确质量全谱采集数据。在实验室规模的实验中评估了 UV/TiO 系统对海水中 OA 的降解效果，并根据 UPLC-QTOF-MS 分析获得的数据提出了转化产物的鉴定。采用磷酸酶抑制试验研究了 UV/TiO 系统的解毒潜力。通过简单的反相快速色谱法，在室内制备了足够量的高纯度 OA（25mg，纯度>90%），用于光催化实验。在 30min 和 7.5min 后，在去离子水中观察到 OA 完全降解。用拟一级动力学模型拟合的速率常数（R>0.96）。用高分辨率质谱对光催化 OA 进行分析，初步鉴定了四个转化产物。在去离子水和人工海水（≤20min）中，OA 的降解与解毒同时进行，但在海水中则不然。总的来说，结果表明 UV/TiO 光催化可以有效地降解海洋环境中的 OA 及其 TPs。据我们所知，这是首次报道用光催化降解海洋毒素及其在保护贝类捕捞区方面的潜在应用。
Zotero 插件
