双碱基编辑器在人类细胞中催化胞嘧啶和腺嘌呤碱基的转换。
Dual base editor catalyzes both cytosine and adenine base conversions in human cells.
机构信息
Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.
Cipher Gene LLC, Beijing, China.
出版信息
Nat Biotechnol. 2020 Jul;38(7):856-860. doi: 10.1038/s41587-020-0527-y. Epub 2020 Jun 1.
Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax's activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.
虽然碱基编辑器是精确基因组编辑的有用工具，但目前的碱基编辑器只能转换腺嘌呤或胞嘧啶。我们通过融合两种脱氨酶和 Cas9 切口酶来开发了一种双腺嘌呤和胞嘧啶碱基编辑器（A&C-BEmax），以在同一靶位点实现 C 到 T 和 A 到 G 的转换。与单碱基编辑器相比，A&C-BEmax 对腺嘌呤的活性略有降低，而对胞嘧啶的活性更高，并且 RNA 脱靶活性大大降低。
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