P-MAPA免疫调节剂可部分预防寨卡病毒感染诱导的THP-1细胞凋亡。
The P-MAPA Immunomodulator Partially Prevents Apoptosis Induced by Zika Virus Infection in THP-1 Cells.
作者信息
Lima Morganna C, Azevedo Elisa A N, de Morais Clarice N L, de Sousa Larissa I O, Carvalho Bruno M, da Silva Iseu N, Franca Rafael F O
机构信息
Department of Virology and Experimental Therapy, Aggeu Magalhaes Institute, Oswaldo Cruz Foundation/FIOCRUZ, Recife, Brazil.
Cesmac University Center, Maceio, Brazil.
出版信息
Curr Pharm Biotechnol. 2021;22(4):514-522. doi: 10.2174/1389201021666200602140005.
BACKGROUND
Zika virus is an emerging arbovirus of global importance. ZIKV infection is associated with a range of neurological complications such as the Congenital Zika Syndrome and Guillain Barré Syndrome. Despite the magnitude of recent outbreaks, there is no specific therapy to prevent or to alleviate disease pathology.
OBJECTIVE
To investigate the role of P-MAPA immunomodulator in Zika-infected THP-1 cells.
METHODS
THP-1 cells were subjected to Zika virus infection (Multiplicity of Infection = 0.5) followed by treatment with P-MAPA for until 96 hours post-infection. After that, the cell death was analyzed by annexin/ PI and caspase 3/ 7 staining by flow cytometry. In addition, virus replication and cell proliferation were accessed by RT-qPCR and Ki67 staining, respectively.
RESULTS
We demonstrate that P-MAPA in vitro treatment significantly reduces Zika virus-induced cell death and caspase-3/7 activation on THP-1 infected cells, albeit it has no role in virus replication and cell proliferation.
CONCLUSION
Our study reveals that P-MAPA seems to be a satisfactory alternative to inhibit the effects of Zika virus infection in mammalian cells.
背景
寨卡病毒是一种新出现的具有全球重要性的虫媒病毒。寨卡病毒感染与一系列神经并发症相关，如先天性寨卡综合征和吉兰-巴雷综合征。尽管近期疫情规模巨大，但尚无预防或减轻疾病病理的特异性疗法。
目的
研究P-MAPA免疫调节剂在寨卡病毒感染的THP-1细胞中的作用。
方法
用寨卡病毒感染THP-1细胞（感染复数=0.5），然后用P-MAPA处理至感染后96小时。之后，通过膜联蛋白/碘化丙啶和流式细胞术检测caspase 3/7染色分析细胞死亡情况。此外，分别通过逆转录定量聚合酶链反应和Ki67染色评估病毒复制和细胞增殖情况。
结果
我们证明，体外使用P-MAPA处理可显著减少寨卡病毒诱导的THP-1感染细胞的细胞死亡和caspase-3/7激活，尽管其对病毒复制和细胞增殖无作用。
结论
我们的研究表明，P-MAPA似乎是抑制寨卡病毒感染对哺乳动物细胞影响的一种令人满意的替代方法。
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