Dept. of Scienze della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
Dept. of Scienze della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
Colloids Surf B Biointerfaces. 2020 Sep;193:111146. doi: 10.1016/j.colsurfb.2020.111146. Epub 2020 May 24.
Grape extract-loaded fibre-enriched vesicles, nutriosomes, were prepared by combining antioxidant extracts obtained from grape pomaces and a prebiotic, soluble fibre (Nutriose®FM06). The nutriosomes were small in size (from ∼140 to 260 nm), homogeneous (polydispersity index < 0.2) and highly negative (∼ -79 mV). The vesicles were highly stable during 12 months of storage at 25 °C. When diluted with warmed (37 °C) acidic medium (pH 1.2) of high ionic strength, the vesicles only displayed an increase of the mean diameter and a low release of the extract, which were dependent on Nutriose concentration. The formulations were highly biocompatible and able to protect intestinal cells (Caco-2) from oxidative stress damage. In vivo results underlined that the composition of mouse microbiota was not affected by the vesicular formulations. Overall results support the potential application of grape nutriosomes as an alternative strategy for the protection of the intestinal tract.
葡萄提取物负载的富含纤维的囊泡，即营养体，是通过将从葡萄渣中获得的抗氧化提取物与一种益生元、可溶性纤维（Nutriose®FM06）结合而制备的。这些营养体体积小（约 140 至 260nm），均一（多分散指数<0.2）且高度负电性（约-79mV）。在 25°C 下储存 12 个月时，囊泡非常稳定。当用加热（37°C）的酸性介质（pH 1.2）高离子强度稀释时，囊泡仅显示平均直径的增加和提取物的低释放，这取决于 Nutriose 的浓度。这些配方具有高度的生物相容性，能够保护肠道细胞（Caco-2）免受氧化应激损伤。体内结果表明，囊泡制剂不会影响小鼠肠道菌群的组成。总体结果支持将葡萄营养体作为保护肠道的替代策略的潜在应用。
