禽β-防御素5对鸡巨噬细胞系的免疫调节作用
Immunomodulatory effects of avian β-defensin 5 in chicken macrophage cell line.
作者信息
Hong Yeojin, Lee Jiae, Vu Thi Hao, Lee Sooyeon, Lillehoj Hyun S, Hong Yeong Ho
机构信息
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
Animal Biosciences and Biotechnology Laboratory, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD 20705, USA.
出版信息
Res Vet Sci. 2020 Oct;132:81-87. doi: 10.1016/j.rvsc.2020.06.002. Epub 2020 Jun 5.
Antimicrobial peptides (AMPs) protect host from pathogens as first line of defense. Especially, β-defensins shows antimicrobial activity and immune modulation effects. Avian species also have β-defensins as avian β-defensins (AvBDs) from AvBD1 to AvBD14. In this study, we characterized chicken AvBD5 and demonstrated its immune modulatory functions in chicken macrophage cell line (HD11). Chicken AvBD5 is composed of a signal, pro, and mature peptides containing one α-helix, four β-sheet, and three disulfide bonds. Here, we also showed that chicken AvBD5 induced Th1, Th2, and Th17 cytokines in chicken macrophage cell line and stimulated MAPK signaling pathways through ERK1/2 and p38 molecules. In addition, AvBD5 stimulated MyD88 and CD40 to regulate immune systems. Taken together, chicken AvBD5 can modulate host immune systems by inducing cytokines expression and stimulating MAPK signaling pathway.
抗菌肽（AMPs）作为第一道防线保护宿主免受病原体侵害。特别是，β-防御素具有抗菌活性和免疫调节作用。禽类也有β-防御素，即从禽β-防御素1（AvBD1）到禽β-防御素14（AvBD14）。在本研究中，我们对鸡AvBD5进行了表征，并证明了其在鸡巨噬细胞系（HD11）中的免疫调节功能。鸡AvBD5由信号肽、前肽和成熟肽组成，含有一个α-螺旋、四个β-折叠和三个二硫键。在此，我们还表明鸡AvBD5在鸡巨噬细胞系中诱导Th1、Th2和Th17细胞因子，并通过ERK1/2和p38分子刺激丝裂原活化蛋白激酶（MAPK）信号通路。此外，AvBD5刺激髓样分化因子88（MyD88）和CD40来调节免疫系统。综上所述，鸡AvBD5可通过诱导细胞因子表达和刺激MAPK信号通路来调节宿主免疫系统。
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