一种硫醇化含砷碳氢化合物的细胞毒理学特征
Cellular toxicological characterization of a thioxolated arsenic-containing hydrocarbon.
作者信息
Ebert Franziska, Ziemann Vanessa, Wandt Viktoria Klara, Witt Barbara, Müller Sandra Marie, Guttenberger Nikolaus, Bankoglu Ezgi Eyluel, Stopper Helga, Raber Georg, Francesconi Kevin A, Schwerdtle Tanja
机构信息
University of Potsdam, Institute of Nutritional Science, Department of Food Chemistry, Arthur-Scheunert-Allee 114-116, Nuthetal, Germany.
University of Potsdam, Institute of Nutritional Science, Department of Food Chemistry, Arthur-Scheunert-Allee 114-116, Nuthetal, Germany.
出版信息
J Trace Elem Med Biol. 2020 Sep;61:126563. doi: 10.1016/j.jtemb.2020.126563. Epub 2020 May 24.
Arsenolipids, especially arsenic-containing hydrocarbons (AsHC), are an emerging class of seafood originating contaminants. Here we toxicologically characterize a recently identified oxo-AsHC 332 metabolite, thioxo-AsHC 348 in cultured human liver (HepG2) cells. Compared to results of previous studies of the parent compound oxo-AsHC 332, thioxo-AsHC 348 substantially affected cell viability in the same concentration range but exerted about 10-fold lower cellular bioavailability. Similar to oxo-AsHC 332, thioxo-AsHC 348 did not substantially induce oxidative stress nor DNA damage. Moreover, in contrast to oxo-AsHC 332 mitochondria seem not to be a primary subcellular toxicity target for thioxo-AsHC 348. This study indicates that thioxo-AsHC 348 is at least as toxic as its parent compound oxo-AsHC 332 but very likely acts via a different mode of toxic action, which still needs to be identified.
砷脂类，尤其是含砷碳氢化合物（AsHC），是一类新出现的源自海产品的污染物。在此，我们对一种最近鉴定出的含氧-AsHC 332代谢产物——硫代-AsHC 348，在培养的人肝癌（HepG2）细胞中进行了毒理学特性研究。与先前对母体化合物含氧-AsHC 332的研究结果相比，硫代-AsHC 348在相同浓度范围内对细胞活力有显著影响，但细胞生物利用度约低10倍。与含氧-AsHC 332相似，硫代-AsHC 348并未显著诱导氧化应激或DNA损伤。此外，与含氧-AsHC 332不同，线粒体似乎不是硫代-AsHC 348的主要亚细胞毒性靶点。本研究表明，硫代-AsHC 348的毒性至少与其母体化合物含氧-AsHC 332相当，但很可能通过不同的毒性作用模式起作用，其具体机制仍有待确定。
Zotero 插件