Assinder S J, Williams P A
Department of Biochemistry, School of Biological Sciences, University College of North Wales, Bangor, UK.
J Gen Microbiol. 1988 Oct;134(10):2769-78. doi: 10.1099/00221287-134-10-2769.
The regulated meta pathway operon for the catabolism of salicylate on the naphthalene plasmid pWW60-22 was cloned into the broad-host-range vector pKT230 on a 17.5 kbp BamHI fragment. The recombinant plasmid conferred the ability to grow on salicylate when mobilized into plasmid-free Pseudomonas putida PaW130. A detailed restriction map of the insert was derived and the locations of some of the genes were determined by subcloning and assaying for their gene products in Escherichia coli and P. putida hosts. The existence of a regulatory gene was demonstrated by the induction of enzyme activities in the presence of salicylate. DNA-DNA hybridization indicated a high degree of structural homology between the pWW60-22 operon and the analogous meta pathway operon on TOL plasmid pWW53-4. The data are consistent with the structural genes being arranged in an identical linear array and suggest an evolutionary link between the two catabolic systems.
萘质粒pWW60 - 22上用于水杨酸酯分解代谢的调控间位途径操纵子，以一个17.5 kbp的BamHI片段被克隆到广宿主范围载体pKT230中。当重组质粒转入无质粒的恶臭假单胞菌PaW130时，赋予了其在水杨酸酯上生长的能力。推导了插入片段的详细限制酶切图谱，并通过亚克隆以及在大肠杆菌和恶臭假单胞菌宿主中对其基因产物进行检测，确定了一些基因的位置。通过在水杨酸酯存在下酶活性的诱导，证明了调控基因的存在。DNA - DNA杂交表明pWW60 - 22操纵子与TOL质粒pWW53 - 4上类似的间位途径操纵子之间存在高度的结构同源性。数据表明结构基因以相同的线性排列方式排布，提示这两个分解代谢系统之间存在进化联系。
