2020 年 3 月德国基于定量 PCR 检测 SARS-CoV-2 的多中心比较。

Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020.

机构信息

Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.

出版信息

Euro Surveill. 2020 Jun;25(24). doi: 10.2807/1560-7917.ES.2020.25.24.2001057.

DOI:10.2807/1560-7917.ES.2020.25.24.2001057

PMID:32583765

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7315722/

Abstract

Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic.

摘要

在当前大流行期间，对冠状病毒病 (COVID-19) 患者的控制策略和临床管理取决于对严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的可靠诊断 PCR 检测。在这里，我们比较了 2020 年 3 月德国七个诊断实验室使用的 11 种不同的 RT-PCR 检测系统。虽然大多数检测方法都表现良好，但我们发现一种常用检测方法存在检测问题，这可能导致大流行的最初几周内出现假阴性检测结果。