Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, People's Republic of China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; College of Forestry, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
Bioresour Technol. 2020 Oct;313:123705. doi: 10.1016/j.biortech.2020.123705. Epub 2020 Jun 18.
Furfuryl alcohol is an important reduction product from biomass derived furfural. This study developed one-pot biosynthesis of furfuryl alcohol and lactic acid by a glucose coupled biphasic system using single Bacillus coagulans NL01. Water/dioctyl phthalate is chosen as biphasic system to alleviate the toxicity of furfural and furfuryl alcohol. Under the optimal conditions, the high-concentration conversion (208 mM) of furfural was successfully converted in 6 h reaction with 98% furfural conversion and 88% furfuryl alcohol selectivity. Notably, glucose as co-substrate could be effectively converted to lactic acid in this biphasic system. About 264 mM furfuryl alcohol and 64.2 g/L lactic acid were simultaneously produced from 310 mM furfural and 71.3 g/L glucose within 8.5 h by a fed-batch strategy. The developed approach can not only increase the produced furfuryl alcohol concentration but also reduce the cost of overall approach by lactic acid co-production, indicating its potential for industrial applications.
糠醇是由生物质衍生的糠醛的重要还原产物。本研究利用一株凝结芽孢杆菌 NL01，通过葡萄糖偶联的双相体系，开发了一锅法同时合成糠醇和乳酸的方法。选择水/邻苯二甲酸二辛酯作为双相体系，以减轻糠醛和糠醇的毒性。在最佳条件下，成功地将高浓度（208mM）的糠醛在 6h 反应中转化，糠醛转化率为 98%，糠醇选择性为 88%。值得注意的是，葡萄糖作为共底物可以在该双相体系中有效地转化为乳酸。通过分批补料策略，从 310mM 糠醛和 71.3g/L 葡萄糖中，在 8.5h 内同时生产出 264mM 糠醇和 64.2g/L 乳酸。该方法不仅可以提高糠醇的浓度，还可以通过联产乳酸降低总体方法的成本，表明其在工业应用上具有潜力。
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