人肝细胞在 PiZ-NSG 小鼠模型中的植入。
Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model.
机构信息
Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA.
Department of Pediatrics, Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA.
出版信息
Methods Mol Biol. 2020;2164:75-85. doi: 10.1007/978-1-0716-0704-6_9.
We recently described the generation of a novel mouse strain that efficiently and readily engrafts human primary hepatocytes to create liver xenografts (Borel et al., Mol Ther, 25: 2477-89, 2017). A transgenic mouse strain expressing a human PiZ allele for the SerpinA1 gene was crossed with the NOD-SCID-gamma chain knockout (NSG) strain to create a recipient strain (PiZ-NSG) for human hepatocyte xenotransplantation. In this chapter we provide a description of the methods to achieve these liver xenografts in the PiZ-NSG mouse.
我们最近描述了一种新型小鼠品系的产生，该品系能够高效且容易地植入人原代肝细胞，从而创建肝异种移植（Borel 等人，Mol Ther，25：2477-89，2017）。一种表达人丝氨酸蛋白酶抑制剂 A1 基因 PiZ 等位基因的转基因小鼠品系与 NOD-SCID-γ 链敲除（NSG）品系杂交，以创建用于人肝细胞异种移植的受体品系（PiZ-NSG）。在本章中，我们提供了在 PiZ-NSG 小鼠中实现这些肝异种移植的方法描述。
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