Effect of bioactive compounds extracted from ANDES-F1080 on laccase activity of ANDES-F515.

Laccases are ligninolytic enzymes produced by different microorganisms, especially by fungi such as the white-rot fungus . Chemical inductors have been used to promote laccase secretion due to the application of these enzymes in lignocellulosic biomass pretreatment. ANDES-F1080 was previously described as a source of bioactive compounds that could influence the enzymatic production system of other fungi. For that reason, this study evaluates the effect of ANDES-F1080 extracts on the laccase activity of ANDES-F515. To achieve this objective, ANDES-F1080 was grown in four different substrates: two artificial-based and two natural-based culture media. Metabolites were extracted from ANDES-F1080 using water and methanol as solvents. Biochemical characterization of these extracts was performed to complement the analysis of their effect on laccase activity. Our results revealed an enhancement on the laccase activity of ANDES-F515 grown in natural-based cultures when ANDES-F1080 extracts were supplemented. The best laccase activities registered values around 10,575 ± 813 U·L.