Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; Endometrium and Endometriosis Research Centre, Hamadan University of Medical Sciences, Hamadan, Iran.
Acta Histochem. 2020 Jul;122(5):151556. doi: 10.1016/j.acthis.2020.151556. Epub 2020 May 20.
Asherman's syndrome (AS) is an endometrial damage that results in infertility in women. Although stem cell therapy has been introduced as a potential treatment for this syndrome, its use in clinical settings remains challenging because of the likelihood of contamination and cell differentiation. Herein, we investigated the effects of adipose-derived stromal vascular fraction (SVF) transplantation on proliferation and angiogenesis in the endometrium in an AS model. The AS model was induced using scratch method in adult male Wistar rats, and SVF (5 × 10 (Simsir et al., 2019) cells) was locally administered into the damaged horns. Two weeks after cell transplantation, endometrial thickness, fibrosis, and expression of vascular endothelial growth factor (VEGF) were assessed by Hematoxylin & Eosin, Masson's trichrome, and immunofluorescence staining, respectively. We found thin endometrium, increased fibrosis, and decreased VEGF following AS induction all of which were reversed after SVF transplantation. We concluded that the local injection of SVF may serve as an effective alternative therapy for AS.
Asherman 综合征（AS）是一种子宫内膜损伤，可导致女性不孕。尽管干细胞疗法已被引入作为该综合征的一种潜在治疗方法，但由于污染和细胞分化的可能性，其在临床环境中的应用仍然具有挑战性。在此，我们研究了脂肪来源的基质血管部分（SVF）移植对 AS 模型中子宫内膜增殖和血管生成的影响。通过划痕法在成年雄性 Wistar 大鼠中诱导 AS 模型，并将 SVF（5×10 个细胞）局部注入损伤的角。细胞移植后 2 周，通过苏木精和伊红、Masson 三色和免疫荧光染色分别评估子宫内膜厚度、纤维化和血管内皮生长因子（VEGF）的表达。我们发现，AS 诱导后出现子宫内膜薄、纤维化增加和 VEGF 减少，这些均在 SVF 移植后得到逆转。我们得出结论，SVF 的局部注射可能是 AS 的一种有效替代治疗方法。
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