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基于细胞色素C氧化酶I(COI)基因片段对越南承天顺化省花鳗鲡种群的系统发育分析。

Phylogenetic analysis of Anguilla marmorata population in Thua Thien Hue, Vietnam based on the cytochrome C oxidase I (COI) gene fragments.

作者信息

Huyen Kieu Thi, Linh Nguyen Quang

机构信息

Faculty of Fisheries, University of Agriculture and Forestry, Hue University, 102 Phung Hung, Hue, 49000, Vietnam.

Department of Nutritional Diseases and Systems for Livestock and Aquaculture, Institute of Biotechnology, Hue University, Hue City, 49000, Vietnam.

出版信息

AMB Express. 2020 Jul 7;10(1):122. doi: 10.1186/s13568-020-01059-7.

Abstract

The giant mottled eel is a species with high commercial value so overfishing, river management, and water pollution have negatively affected its movement and population numbers. Anguilla marmorata (eel) was listed in the Vietnam Red Data Book 2007 with a description of Vulnerability. This study used a barcode technique to analyze molecular characteristics and build genetic plants based on the cytochrome c oxidase I gene segment isolated from the mitochondrial genome of 48 individuals of A. marmorata collected in five different ecological regions of Thua Thien Hue, Vietnam. The isolated the cytochrome c oxidase I sequence has a length of 843 nucleotides, four base nucleotides of 30.03% Thymine, 25.15% Cytosine, 27.49% Adenine, and 17.43% Guanine. The percentage of Guanine + Cytosine content (42.58%) is acceptable, lower than the Adenine + Thymine content. The replacement capacity of Adenine and Guanine is 22.45% highest, the ratio between Thymine and Guanine; Cytosine and Guanine are the lowest at 2.72%. The establishment of genetically modified plants has shown the high genetic similarity of individuals in eel populations in Thua Thien Hue. The population of Anguilla marmorata eels in Thua Thien Hue, Vietnam is divided into two separate groups that are guided by the migration process and specific ecological. This is particularly important in building strategies to conserve and develop the gene for eel in Vietnam and Thua Thien Hue.

摘要

巨斑花鳗是一种具有高商业价值的物种,因此过度捕捞、河流管理和水污染对其活动及种群数量产生了负面影响。花鳗鲡在《2007年越南红色数据手册》中被列为易危物种。本研究采用条形码技术分析分子特征,并基于从越南承天顺化五个不同生态区域采集的48条花鳗鲡个体的线粒体基因组中分离出的细胞色素c氧化酶I基因片段构建遗传图谱。分离出的细胞色素c氧化酶I序列长度为843个核苷酸,四种碱基核苷酸中,胸腺嘧啶占30.03%,胞嘧啶占25.15%,腺嘌呤占27.49%,鸟嘌呤占17.43%。鸟嘌呤+胞嘧啶含量百分比(42.58%)是可接受的,低于腺嘌呤+胸腺嘧啶含量。腺嘌呤和鸟嘌呤的替换能力最高,为22.45%,胸腺嘧啶与鸟嘌呤、胞嘧啶与鸟嘌呤的比例最低,为2.72%。转基因植物的建立表明承天顺化花鳗种群个体具有高度的遗传相似性。越南承天顺化的花鳗鲡种群分为两个独立的群体,这受洄游过程和特定生态环境的影响。这对于制定越南和承天顺化花鳗基因的保护和开发策略尤为重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdc3/7340690/bfae823ad89d/13568_2020_1059_Fig1_HTML.jpg

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