新型木聚糖酶产生菌X2：分子系统发育分析及其在低聚木糖生产中的应用
Novel xylanase producing strain X2: molecular phylogenetic analysis and its application for production of xylooligosaccharides.
作者信息
Dhruw Chandrabhan, Husain Khadim, Kumar Vyas, Sonawane Vijay Chintaman
机构信息
BERPDC, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036 India.
出版信息
3 Biotech. 2020 Aug;10(8):328. doi: 10.1007/s13205-020-02322-1. Epub 2020 Jul 3.
A strain X2 that produced extracellular endo-xylanase (GH 11) (EC: 3.2.1.8) was isolated from the soil of the Northeast India region. This aerobic culture was Gram positive and endospore forming. Chemotaxonomic characterization showed variance with the fatty acid profile of related species in the group. In strain X2, distinct occurrence of iso-C lipids is absent in other related species. The 16S rRNA gene sequence homology showed 99% similarity with subsp. The phylogenetic analysis by the multilocus sequence analysis (MLSA) of the nucleotide sequence of six concatenated genes (16S rRNA, , , , and ) resolved the taxonomic position of the strain X2 in the subsp. group. The MLSA showed that it is a member of a clade that includes subsp. . In DNA-DNA hybridization (DDH), the highest matching score was obtained with subsp. (87%). The in silico DDH of the genome (G + C 43.7 mol %) shared 48.5%, with subsp. . The MLSA phylogenetic tree and the highest degree of DNA hybridization, indicating that it belongs to the subspecies .
从印度东北地区的土壤中分离出一株产生胞外内切木聚糖酶（GH 11）（EC：3.2.1.8）的X2菌株。该需氧培养物为革兰氏阳性且形成芽孢。化学分类学特征显示与该组相关物种的脂肪酸谱存在差异。在X2菌株中，其他相关物种不存在异-C脂质的明显出现。16S rRNA基因序列同源性显示与亚种有99%的相似性。通过对六个串联基因（16S rRNA、、、、和）的核苷酸序列进行多位点序列分析（MLSA）的系统发育分析确定了X2菌株在亚种组中的分类地位。MLSA表明它是包括亚种的一个进化枝的成员。在DNA-DNA杂交（DDH）中，与亚种获得了最高匹配分数（87%）。基因组的电子DDH（G + C 43.7 mol%）与亚种共享48.5%。MLSA系统发育树和最高程度的DNA杂交表明它属于亚种。
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