González Carol, González Daniela, Zúñiga Rommy N, Estay Humberto, Troncoso Elizabeth
Department of Chemistry, Universidad Tecnológica Metropolitana, Las Palmeras 3360, Ñuñoa, Santiago 7800003, Chile.
Department of Biotechnology, Universidad Tecnológica Metropolitana, Las Palmeras 3360, Ñuñoa, Santiago 7800003, Chile.
Foods. 2020 Jul 10;9(7):913. doi: 10.3390/foods9070913.
This work deepens our understanding of starch digestion and the consequent absorption of hydrolytic products generated in the human small intestine. Gelatinized starch dispersions were digested with α-amylase in an in vitro intestinal digestion system (-IDS) based on a dialysis membrane process. This study innovates with respect to the existing literature, because it considers the impact of simultaneous digestion and absorption processes occurring during the intestinal digestion of starchy foods and adopts phenomenological models that deal in a more realistic manner with the behavior found in the small intestine. Operating the -IDS at different flow/dialysate flow ratios resulted in distinct generation and transfer curves of reducing sugars mass. This indicates that the operating conditions affected the mass transfer by diffusion and convection. However, the transfer process was also affected by membrane fouling, a dynamic phenomenon that occurred in the -IDS. The experimental results were extrapolated to the human small intestine, where the times reached to transfer the hydrolytic products ranged between 30 and 64 min, according to the flow ratio used. We consider that the -IDS is a versatile system that can be used for assessing and/or comparing digestion and absorption behaviors of different starch-based food matrices as found in the human small intestine, but the formation and interpretation of membrane fouling requires further studies for a better understanding at physiological level. In addition, further studies with the -IDS are required if food matrices based on fat, proteins or more complex carbohydrates are of interest for testing. Moreover, a next improvement step of the IDS must include the simulation of some physiological events (e.g., electrolytes addition, enzyme activities, bile, dilution and pH) occurring in the human small intestine, in order to improve the comparison with in vivo data.
这项工作加深了我们对淀粉消化以及人类小肠中水解产物后续吸收的理解。在基于透析膜过程的体外肠道消化系统（-IDS）中，用α-淀粉酶消化糊化淀粉分散液。这项研究相对于现有文献有所创新，因为它考虑了淀粉类食物在肠道消化过程中同时发生的消化和吸收过程的影响，并采用了能更真实地处理小肠中发现的行为的现象学模型。以不同的流动/透析液流动比率操作-IDS，得到了还原糖质量的不同生成和转移曲线。这表明操作条件影响了扩散和对流的传质。然而，传质过程也受到膜污染的影响，这是在-IDS中发生的一种动态现象。根据所使用的流动比率，实验结果外推至人类小肠时，水解产物的转移时间在30至64分钟之间。我们认为-IDS是一个通用系统，可用于评估和/或比较人类小肠中不同淀粉基食物基质的消化和吸收行为，但膜污染的形成和解释需要进一步研究，以便在生理水平上有更好的理解。此外，如果对基于脂肪、蛋白质或更复杂碳水化合物的食物基质进行测试感兴趣，则需要对-IDS进行进一步研究。而且，IDS的下一个改进步骤必须包括模拟人类小肠中发生的一些生理事件（例如添加电解质、酶活性、胆汁、稀释和pH值），以改善与体内数据的比较。
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