鉴定印度芥菜 Brassica juncea 中 STP4 启动子作为蚜虫响应启动子的特性。
Characterization of STP4 promoter in Indian mustard Brassica juncea for use as an aphid responsive promoter.
机构信息
ICAR-National Institute for Plant Biotechnology, ICAR-Indian Agricultural Research Institute Campus, New Delhi, 110012, India.
Division of Plant Physiology, ICAR-Indian Agricultural Research Institute Campus, New Delhi, 110012, India.
出版信息
Biotechnol Lett. 2020 Oct;42(10):2013-2033. doi: 10.1007/s10529-020-02961-7. Epub 2020 Jul 16.
OBJECTIVE
Brassica juncea, a major oilseed crop, suffers substantial yield losses due to infestation by mustard aphids (Lipaphis erysimi). Unavailability of resistance genes within the accessible gene pool underpins significance of the transgenic strategy in developing aphid resistance. In this study, we aimed for the identification of an aphid-responsive promoter from B. juncea, based on the available genomic resources.
RESULTS
A monosaccharide transporter gene, STP4 in B. juncea was activated by aphids and sustained increased expression as the aphids colonized the plants. We cloned the upstream intergenic region of STP4 and validated its stand-alone aphid-responsive promoter activity. Further, deletion analysis identified the putative cis-elements important for the aphid responsive promoter activity.
CONCLUSION
The identified STP4 promoter can potentially be used for driving high level aphid-inducible expression of transgenes in plants. Use of aphid-responsive promoter instead of constitutive promoters can potentially reduce the metabolic burden of transgene-expression on the host plant.
目的
芥菜型油菜是一种重要的油料作物，由于受到菜蚜的侵害，其产量损失很大。可利用的基因库中缺乏抗性基因，这凸显了转基因策略在开发抗蚜性方面的重要性。本研究旨在利用现有的基因组资源，从芥菜型油菜中鉴定出一个对蚜虫有反应的启动子。
结果
在芥菜型油菜中，一个单糖转运蛋白基因 STP4 被蚜虫激活，并随着蚜虫在植物上定殖而持续表达上调。我们克隆了 STP4 的上游基因间区，并验证了其独立的蚜虫响应启动子活性。进一步的缺失分析确定了对蚜虫响应启动子活性很重要的假定顺式作用元件。
结论
所鉴定的 STP4 启动子可用于在植物中驱动高水平的蚜虫诱导型转基因表达。使用蚜虫响应启动子而不是组成型启动子，可能会降低转基因表达对宿主植物的代谢负担。
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