利用（CRISPR-Cas9）对土拉弗朗西斯菌进行基因组编辑。
Genome editing of Francisella tularensis using (CRISPR-Cas9).
机构信息
Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK.
Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK.
出版信息
J Microbiol Methods. 2020 Sep;176:106004. doi: 10.1016/j.mimet.2020.106004. Epub 2020 Jul 18.
The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system is a powerful tool for gene editing in eukaryotic genomes but is still being developed for editing bacterial genomes. Here we describe the construction of an all-in-one vector for generating potentially scarless deletion mutants in Francisella tularensis LVS using a CRISPR-Cas9-based system.
簇状规律间隔短回文重复序列 (CRISPR)-CRISPR 相关蛋白 9 (Cas9) 系统是真核基因组基因编辑的强大工具，但仍在开发用于编辑细菌基因组的工具。在这里，我们描述了一种基于 CRISPR-Cas9 系统的用于在弗朗西斯氏菌 LVS 中生成潜在无痕缺失突变体的一体式载体的构建。
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