Lentzea indica sp. nov., a novel actinobacteria isolated from Indian Himalayan-soil.

A novel actinobacterium, strain PSKA42 was isolated from a soil sample of Kashmir Himalaya, India (latitude: 34°-01' N; longitude: 74°-47' E; altitude: 5328 ft). The morphological, biochemical and molecular characteristics predicted that PSKA42 belongs to the genera Lentzea. Using the Ezbiocloud server it has also been manifested that the 16S rRNA gene sequence of PSKA42 was 98.4% similar with Lentzea guizhouensis DHSC013, Lentzea albidocapillata subsp. violacea IMSNU50388; 98.26% similar with Lentzea aerocolonigenes NRRLB-3298, Lentzea albidocapillata subsp. albidocapillata NRRLB-24057 and 98.19% similar with Lentzea californiensis DSM43393. Whole-genome average nucleotide identity values between PSKA42 and L. guizhouensis DHSC013, L. albidocapillata subsp. violaera IMSNU50388, L. aerocolonigenes NRRLB-3298, L. albidocapillata subsp. albidocapillata NRRLB-24057, L. californiensis DSM43393, L. flaviverucosa As40578 were 84.94%, 85.8%, 87.15%, 87.71%, 79.29, and 87.22%, respectively, suggesting that PSKA42 represented a new species. In-silco genome-to-genome distances analysis and MLSA with selected housekeeping genes also indicated that the isolate should be assigned to a new species under the genus Lentzea. Analysis of the whole-cell hydrolysate found mannose and ribose as major sugar while cell wall amino acid was identified as meso-diaminopimelic acid. Major fatty acids (> 5%) were observed as 15:0 iso (23.81%), and 16:0 iso (26.11%). PSKA42 contains polyketide synthase type-I genes but unable to show antimicrobial activity in laboratory conditions. The average GC content in its genome is 68.3%. Further, based on physicochemical, chemotaxonomic markers and molecular analysis, PSKA42 can be assigned as novel species of Lentzea, we proposed Lentzea indica sp nov. The type strain is PSKA42 (JCM 33729, MTCC 12936, MCC 4127).