源自携带KCNA5基因c.775G>A突变的家族性心房颤动患者的患者特异性诱导多能干细胞（ZZUSAHi001-A）的生成。
Generation of patient-specific induced pluripotent stem cells (ZZUSAHi001-A) derived from a familial atrial fibrillation patient carrying KCNA5 c.775G>A mutation.
作者信息
Niu Shaohui, Xiong Haiyan, Li Yuan, Zhang Lihua
机构信息
Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
出版信息
Stem Cell Res. 2020 Aug;47:101915. doi: 10.1016/j.scr.2020.101915. Epub 2020 Jul 15.
Peripheral blood mononuclear cells (PBMCs) were isolated from a 57-year-old female who was clinically diagnosed as familial atrial fibrillation and were reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, SOX2, KLF4 and cMYC. The generated human induced pluripotent stem cell (hiPSC) line, ZZUSAHi001-A, exhibited a normal karyotype, showed robust expression of pluripotency markers and differentiated towards three germ layers in vivo.
外周血单个核细胞（PBMCs）取自一名57岁临床诊断为家族性心房颤动的女性，使用含有重编程因子OCT4、SOX2、KLF4和cMYC的非整合仙台病毒载体对其进行重编程。所产生的人诱导多能干细胞（hiPSC）系ZZUSAHi001-A具有正常的核型，多能性标志物表达强劲，并在体内向三个胚层分化。
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