优化的人心肌细胞重编程方案

Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599, USA.

McAllister Heart Institute, University of North Carolina, Chapel Hill, NC 27599, USA.

STAR Protoc. 2020 Jun 19;1(1). doi: 10.1016/j.xpro.2019.100010. Epub 2020 Jun 3.

Direct cardiac reprogramming, the conversion of fibroblasts into cardiomyocyte-like cells (iCMs), is an attractive approach to heal the injured heart. Here we present a new approach to human cardiac reprogramming that utilizes a polycistronic three-factor reprogramming cocktail and one microRNA. Our protocol produces cardiac Troponin T positive human iCMs (hiCMs) at an efficiency of 40%-60%, approximately double that of previous protocols, within just 2 weeks. The resulting hiCMs display cardiomyocyte-like sarcomere structure, gene expression, and calcium oscillation. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2019).

直接心脏重编程，即将成纤维细胞转化为心肌细胞样细胞（iCMs），是一种有吸引力的方法来修复受损的心脏。在这里，我们提出了一种新的人类心脏重编程方法，该方法利用多顺反子三因素重编程鸡尾酒和一种 microRNA。我们的方案在短短 2 周内以 40%-60%的效率产生心肌肌钙蛋白 T 阳性的人类 iCMs（hiCMs），大约是以前方案的两倍。所得的 hiCMs 显示出类似于心肌细胞的肌节结构、基因表达和钙振荡。有关此方案的使用和执行的完整详细信息，请参阅 Zhou 等人。（2019 年）。