新属，新种，...科的一个新属，从鞘翅目昆虫食塑幼虫的肠道中分离得到。

gen. nov., sp. nov., a novel genus of the family , isolated from the gut of plastic-eating larvae of the Coleoptera insect .

机构信息

Department of Biology, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.

Key Laboratory of Molecular Medicine and Biotherapy, Beijing Institute of Technology, Beijing 100081, PR China.

出版信息

Int J Syst Evol Microbiol. 2020 Sep;70(9):4951-4959. doi: 10.1099/ijsem.0.004364.

DOI:10.1099/ijsem.0.004364

PMID:32744988

Abstract

A bacterial strain, BIT-B35, was isolated from the gut of plastic-eating larvae of the Coleoptera insect . Its taxonomic position was determined by using a polyphasic approach. Cells were white-pigmented, Gram-stain-negative, motile short rods with terminal flagella. The 16S rRNA gene sequence (1411 bp) of strain BIT-B35 showed highest similarity (98.1%) to ATCC 35469 and LMG 5519. The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of seven housekeeping genes (, , , , , and ) and genome sequences, placed strain BIT-B35 in a separate lineage among the family of . The major fatty acids were C, C cyclo and C cyclo 8. The genomic DNA G+C content of strain BIT-B35 was 57.1 mol%. The chemotaxonomic data plus results of physiological and biochemical tests also distinguished strain BIT-B35 from members of other genera within the family . Therefore, strain BIT-B35 is considered to represent a novel species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is BIT-B35 (=CGMCC 1.17042=KCTC 72448).

摘要

从鞘翅目昆虫食塑幼虫的肠道中分离到一株细菌菌株 BIT-B35。采用多相分类方法确定其分类地位。细胞呈白色素，革兰氏阴性，运动性短杆状，有端生鞭毛。菌株 BIT-B35 的 16S rRNA 基因序列（1411bp）与 ATCC 35469 和 LMG 5519 的相似度最高（98.1%）。基于 16S rRNA 基因、七个看家基因（、、、、、和）的串联序列和基因组序列的系统发育分析结果，将菌株 BIT-B35 置于科的一个单独分支中。主要脂肪酸为 C、C 环和 C 环 8。菌株 BIT-B35 的基因组 DNA G+C 含量为 57.1mol%。化学分类数据加上生理生化试验结果也将菌株 BIT-B35 与科内其他属的成员区分开来。因此，菌株 BIT-B35 被认为代表了科内一个新属的一个新种，建议使用新属名 gen. nov.，新种名 sp. nov.。模式菌株为 BIT-B35（=CGMCC 1.17042=KCTC 72448）。