沉默调节蛋白-1和组蛋白去乙酰化酶-9在冠状动脉疾病中的反向表达模式
Reverse expression pattern of sirtuin-1 and histone deacetylase-9 in coronary artery disease.
作者信息
Heidari Laleh, Ghaderian Sayyed Mohammad Hossein, Bastami Milad, Hosseini Shadi, Alipour Parsa Saeed, Heidari Sahel, Jafari Hossein, Sohrabifar Nasim, Pirhoushiaran Maryam
机构信息
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Arch Physiol Biochem. 2023 Feb;129(1):46-53. doi: 10.1080/13813455.2020.1797100. Epub 2020 Aug 6.
BACKGROUND
and 9 genes are related to inflammation and may contribute to the pathogenesis of coronary artery disease (CAD). We aimed to evaluate the expression level, methylation profile and polymorphisms of these genes in CAD patients.
METHODS
In this study, 50 CAD patients and 50 healthy individuals were recruited. The expression level change was evaluated using the TaqMan Real-Time PCR method. The methylation of genes promoter and genotyping of polymorphisms were evaluated by the HRM.
RESULTS
The expression level of was reduced while the expression level showed a significant elevation ( < .001). The gene promoter was hypomethylated and the gene promoter was hypermethylated in CAD patients. Also, CG + GG genotype in and both genotypes in the gene were associated with expression change.
CONCLUSIONS
and genes, expression changes can be suggested as a potential biomarker for CAD detection.
背景
9个基因与炎症相关，可能在冠状动脉疾病（CAD）的发病机制中起作用。我们旨在评估这些基因在CAD患者中的表达水平、甲基化谱和多态性。
方法
本研究招募了50例CAD患者和50名健康个体。使用TaqMan实时荧光定量PCR方法评估表达水平变化。通过高分辨率熔解曲线分析（HRM）评估基因启动子的甲基化和多态性基因分型。
结果
的表达水平降低，而 的表达水平显著升高（ < 0.001）。CAD患者中 基因启动子低甲基化， 基因启动子高甲基化。此外， 中的CG + GG基因型和 基因中的两种基因型均与表达变化相关。
结论
和 基因的表达变化可作为CAD检测的潜在生物标志物。
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