Bok S H, Casida L E
Appl Environ Microbiol. 1977 May;33(5):1085-91. doi: 10.1128/aem.33.5.1085-1091.1977.
A screening procedure was used to isolate from soil a Penicillium sp., two bacterial isolates, and a Streptomyces sp. that produced a new microbial growth factor. This factor was an absolute growth requirement for three soil bacteria. The Penicillium sp. and one of the bacteria requiring the factor, an Arthrobacter sp., were selected for more extensive study concerning the production and characteristics of the growth factor. It did not seem to be related to the siderochromes. It was not present in soil extract, rumen fluid, or any other medium component tested. It appears to be a glycoprotein of high molecular weight, and it has high specific activity. When added to the diets for a meadow vole mammalian test system, it caused an increased consumption of diet without a concurrent increase in rate of weight gain.
采用一种筛选程序从土壤中分离出一种青霉菌、两种细菌分离物和一种链霉菌,它们产生了一种新的微生物生长因子。这种因子是三种土壤细菌生长所绝对必需的。选择青霉菌和需要该因子的一种细菌(节杆菌)进行更广泛的研究,以探讨生长因子的产生和特性。它似乎与铁色素无关。在土壤提取物、瘤胃液或测试的任何其他培养基成分中均未发现它。它似乎是一种高分子量糖蛋白,具有高比活性。当添加到草地田鼠哺乳动物测试系统的日粮中时,它导致日粮消耗量增加,但体重增加率没有同时增加。