First Genomic Characterization of and Coharbouring Isolated from Food of Animal Origin.

We describe here the complete genome sequence of an ST279 coharbouring and recovered from uncooked beef patty in June 2017, Egypt. The tested isolate was resistant to carbapenem but susceptible to colistin (minimum inhibitory concentration (MIC), 0.5 μg/mL). The antimicrobial susceptibility profile and conjugation experiments were performed. The entire genome was sequenced by the Illumina MiniSeq and Oxford Nanopore methods. The and genes are carried on the same IncHI2/pMLST1 plasmid, pMS37a (Size of 270.9 kb). The gene was located within the physical boundaries demarcated by two insertion elements IS (upstream) and IS (downstream) but did not possess the downstream regulatory genes (/) which regulate the expression of . Therefore, the might be silently disseminated among carbapenem-resistant Enterobacterales. In addition to and , plasmid pMS37a harbored various antibiotic resistance genes including , Δ, , , To the best of our knowledge, this is the first report of a and -coharbouring isolate of food origin worldwide. The identification of a multidrug-resistant VIM-1 and positive isolate from food is worrisome as retail meat and meat products could serve as a vehicle for these MDR bacteria, which could be transferred between animals and humans through the food chain. It further highlights that Enterobacterales co-producing MCR and carbapenemases being found in the food chain indeed correspond to a One-Health issue, highlighting the need for serious steps to prevent their further dissemination.