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纤毛门(Spirotrichea)内弯尾水螅(Schmidingerella arcuata)的基因组和转录组联合分析揭示了 DNA 消除、重排和倒位的模式。

Combined Genome and Transcriptome Analyses of the Ciliate Schmidingerella arcuata (Spirotrichea) Reveal Patterns of DNA Elimination, Scrambling, and Inversion.

机构信息

Department of Marine Sciences, University of Connecticut, Groton.

Institute of Cell Biology, University of Bern, Switzerland.

出版信息

Genome Biol Evol. 2020 Sep 1;12(9):1616-1622. doi: 10.1093/gbe/evaa185.

DOI:10.1093/gbe/evaa185
PMID:32870974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7523726/
Abstract

Schmidingerella arcuata is an ecologically important tintinnid ciliate that has long served as a model species in plankton trophic ecology. We present a partial micronuclear genome and macronuclear transcriptome resource for S. arcuata, acquired using single-cell techniques, and we report on pilot analyses including functional annotation and genome architecture. Our analysis shows major fragmentation, elimination, and scrambling in the micronuclear genome of S. arcuata. This work introduces a new nonmodel genome resource for the study of ciliate ecology and genomic biology and provides a detailed functional counterpart to ecological research on S. arcuata.

摘要

弯尾钟虫(Schmidingerella arcuata)是一种生态上重要的有孔虫纤毛虫,长期以来一直作为浮游动物营养生态学的模式物种。我们使用单细胞技术为 S. arcuata 提供了部分微核基因组和大核转录组资源,并报告了包括功能注释和基因组结构在内的初步分析结果。我们的分析表明,S. arcuata 的微核基因组发生了重大的断裂、消除和重排。这项工作为纤毛虫生态学和基因组生物学的研究引入了新的非模式基因组资源,并为 S. arcuata 的生态学研究提供了详细的功能对应物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e94c/7523726/dcb3e7bf545b/evaa185f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e94c/7523726/dcb3e7bf545b/evaa185f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e94c/7523726/dcb3e7bf545b/evaa185f1.jpg

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mBio. 2019 Dec 24;10(6):e02524-19. doi: 10.1128/mBio.02524-19.
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Ultrastructural Studies on a Model Tintinnid - (Kofoid and Campbell, 1929) Agatha and Strüder-Kypke, 2012 (Ciliophora). I. Somatic Kinetids with Unique Ultrastructure.一种铃壳虫模型的超微结构研究 - (科福伊德和坎贝尔,1929年)阿加莎属及施特吕德 - 基普克,2012年(纤毛门)。I. 具有独特超微结构的体细胞动基体
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Somatic genome architecture and molecular evolution are decoupled in "young" linage-specific gene families in ciliates.
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纤毛虫基因组结构的进化起源和影响。
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