肝癌衍生生长因子刺激诱导的肝癌相关 microRNAs。
Hepatocellular Carcinoma-associated microRNAs Induced by Hepatoma-derived Growth Factor Stimulation.
机构信息
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan
Department of Gastroenterology, Nippon Life Hospital, Osaka, Japan.
出版信息
In Vivo. 2020 Sep-Oct;34(5):2297-2301. doi: 10.21873/invivo.12041.
BACKGROUND/AIM: Hepatoma-derived growth factor (HDGF) is involved in the progression of hepatocellular carcinoma (HCC). The present study assessed the epigenomic changes in hepatoma-derived cells through HDGF stimulation.
MATERIALS AND METHODS
We used two hepatoma-derived cell lines (HepG2 and SK-Hep1) and searched for microRNAs whose expression commonly changed in response to HDGF administration. We further explored a genetic database to investigate the association of the candidate microRNAs with the survival of HCC patients.
RESULTS
Despite both HepG2 and SK-Hep1 cells being categorized as hepatoma-derived cells, the microRNA profile differed between these two lines. However, HepG2 and SK-Hep1 cells shared 30 up-regulated and 2 down-regulated microRNAs. Of these, miR-6072 and miR-3137 were significantly associated with a poor prognosis in HCC patients.
CONCLUSION
We identified two candidate microRNAs whose expression increased in response to HDGF stimulation. Both these molecules were associated with a poor prognosis of HCC patients.
背景/目的：肝癌衍生生长因子（HDGF）参与肝细胞癌（HCC）的进展。本研究通过 HDGF 刺激评估肝癌衍生细胞的表观基因组变化。
材料和方法
我们使用两种肝癌衍生细胞系（HepG2 和 SK-Hep1），并寻找表达普遍因 HDGF 给药而改变的 microRNAs。我们进一步探索了一个遗传数据库，以研究候选 microRNAs 与 HCC 患者生存的关联。
结果
尽管 HepG2 和 SK-Hep1 细胞均被归类为肝癌衍生细胞，但这两种细胞系之间的 microRNA 谱存在差异。然而，HepG2 和 SK-Hep1 细胞共有 30 个上调和 2 个下调的 microRNAs。其中，miR-6072 和 miR-3137 与 HCC 患者的不良预后显著相关。
结论
我们鉴定了两种因 HDGF 刺激而表达增加的候选 microRNAs。这两种分子均与 HCC 患者的不良预后相关。
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