Sahoo Lakshman, Das Paramananda, Sahoo Bismay, Das Gargee, Meher Prem Kumar, Udit Uday Kumar, Mahapatra Kanta Das, Sundaray Jitendra Kumar
Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, Odisha, 751002, India.
BMC Res Notes. 2020 Sep 3;13(1):411. doi: 10.1186/s13104-020-05240-w.
Labeo catla (catla), one of the three Indian major carps, is native to the Indo-Gangetic riverine system of India as well as the rivers of Pakistan, Bangladesh, Nepal and Myanmar. Its higher growth rate and compatibility with other major carps, specific surface feeding habit, and consumer preference have increased its popularity in carp polyculture systems among the fish farmers in Indian subcontinent. Recent advancement in sequencing technology coupled with massive parallel sequencing platforms has facilitated accelerated genetic improvement in aquaculture species through integration of genomics tools. A draft genome and allied resources are lacking in catla. Therefore, in the present study, we have performed de-novo assembly of Labeo catla for the first time.
A male farm reared catla was used for extracting high molecular weight genomic DNA followed by sequencing in Oxford Nanopore and Illumina platforms. Approximately, 80× coverage of sequence data was assembled adopting the hybrid assembly strategy. The assembled genome size of catla was 1.01 Gb containing 5345 scaffolds with N50 value 0.7 Mb and more than 92% BUSCO completeness. Gene annotation resulted in 25,812 predicted genes.
印度三大主要鲤鱼之一的印度鲃(Catla)原产于印度的印度-恒河河系以及巴基斯坦、孟加拉国、尼泊尔和缅甸的河流。其较高的生长速度、与其他主要鲤鱼的兼容性、独特的表层摄食习性以及消费者偏好,使其在印度次大陆的养鱼户中,在鲤鱼混养系统中越来越受欢迎。测序技术的最新进展与大规模平行测序平台相结合,通过整合基因组学工具,促进了水产养殖物种的加速遗传改良。印度鲃缺乏基因组草图和相关资源。因此,在本研究中,我们首次对印度鲃进行了从头组装。
使用一条雄性养殖印度鲃提取高分子量基因组DNA,随后在牛津纳米孔和Illumina平台上进行测序。采用混合组装策略组装了约80倍覆盖度的序列数据。印度鲃组装后的基因组大小为1.01Gb,包含5345个支架,N50值为0.7Mb,BUSCO完整性超过92%。基因注释产生了25812个预测基因。