通过 SCRaMbLE 对合成酵母进行染色体重排。

Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE.

机构信息

CAS Key Laboratory of Quantitative Engineering Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

出版信息

Methods Mol Biol. 2021;2196:153-165. doi: 10.1007/978-1-0716-0868-5_12.

DOI:10.1007/978-1-0716-0868-5_12

PMID:32889719

Abstract

Diversified genomes derived from chromosomal rearrangements are valuable materials for evolution. Naturally, chromosomal rearrangements occur at extremely low frequency to ensure genome stability. In the synthetic yeast genome project (Sc2.0), an inducible chromosome rearrangement system named Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) is built to produce chromosomal rearrangements such as deletion, duplication, inversion, and translocation at high efficiency. Here, we detail the method to activate SCRaMbLE in a synthetic strain, to analyze the SCRaMbLEd genome, and to dissect the causative rearrangements for a desired phenotype after SCRaMbLEing.

摘要

多样化的基因组源于染色体重排，是进化的宝贵材料。自然地，染色体重排在极低的频率下发生，以确保基因组的稳定性。在合成酵母基因组计划（Sc2.0）中，构建了一种可诱导的染色体重排系统，名为通过 LoxP 介导的进化实现的合成染色体重排和修饰（SCRaMbLE），以高效产生缺失、重复、倒位和易位等染色体重排。在这里，我们详细介绍了在合成菌株中激活 SCRaMbLE 的方法，分析了 SCRaMbLEd 基因组，并解析了 SCRaMbLE 后产生所需表型的因果重排。