Song Eun-Jung, Españo Erica, Nam Jeong-Hyun, Kim Jiyeon, Shim Kyu-Suk, Shin Eunju, Park Young In, Lee Chong-Kil, Kim Jeong-Ki
Department of Pharmacy, Korea University College of Pharmacy, Sejong 30019, Korea.
Department of Veterinary Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
Immune Netw. 2020 Jul 9;20(4):e31. doi: 10.4110/in.2020.20.e31. eCollection 2020 Aug.
The effectiveness of current influenza vaccines is considered suboptimal, and 1 way to improve the vaccines is using adjuvants. However, the current pool of adjuvants used in influenza vaccination is limited due to safety concerns. , or aloe, has been shown to have immunomodulatory functions and to be safe for oral intake. In this study, we explored the potential of orally administered processed gel (PAG) as an adjuvant for influenza vaccines in C57BL/6 mice. We first evaluated its adjuvanticity with a split-type pandemic H1N1 (pH1N1) Ag by subjecting the mice to lethal homologous influenza challenge. Oral PAG administration with the pH1N1 Ag increased survival rates in mice to levels similar to those of alum and MF59, which are currently used as adjuvants in influenza vaccine formulations. Similarly, oral PAG administration improved the survival of mice immunized with a commercial trivalent influenza vaccine against lethal homologous and heterologous virus challenge. PAG also increased hemagglutination inhibition and virus neutralization Ab titers against homologous and heterologous influenza strains following immunization with the split-type pH1N1 Ag or the commercial trivalent vaccine. Therefore, this study demonstrates that PAG may potentially be used as an adjuvant for influenza vaccines.
目前流感疫苗的有效性被认为并不理想，而改进疫苗的一种方法是使用佐剂。然而，由于安全问题，目前用于流感疫苗接种的佐剂种类有限。芦荟已被证明具有免疫调节功能，且口服安全。在本研究中，我们探讨了口服加工后的芦荟凝胶（PAG）作为C57BL/6小鼠流感疫苗佐剂的潜力。我们首先通过对小鼠进行致死性同源流感攻击，用裂解型大流行H1N1（pH1N1）抗原评估其佐剂活性。口服PAG与pH1N1抗原一起可将小鼠的存活率提高到与明矾和MF59相似的水平，明矾和MF59目前在流感疫苗配方中用作佐剂。同样，口服PAG可提高用市售三价流感疫苗免疫的小鼠在面对致死性同源和异源病毒攻击时的存活率。在用裂解型pH1N1抗原或市售三价疫苗免疫后，PAG还提高了针对同源和异源流感毒株的血凝抑制和病毒中和抗体效价。因此，本研究表明PAG可能有潜力用作流感疫苗的佐剂。
