苦丁茶三萜类成分分析及其对癌细胞系的抗增殖活性。
Composition of Triterpenoids in and Their Anti-Proliferative Activity on Cancer Cell Lines.
机构信息
Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea.
Food Analysis Team, Busan Metropolitan City Institute of Health and Environment, Busan 46616, Korea.
出版信息
Molecules. 2020 Sep 6;25(18):4066. doi: 10.3390/molecules25184066.
The objective of this study was to determine the composition of triterpenoids in the extracts from the inner and outer parts of and to evaluate their anti-proliferative activity against cancer cell lines (HT-29, AGS, MCF-7, and PC3). Inner and outer parts of were extracted with 80% methanol for 24 h. The extract was fractionated by Diaion HP-20 resin to obtain the triterpenoid fraction. Composition of triterpenoids in the fraction was analyzed by HPLC and LC-ESI-MS. Anti-proliferative activity was evaluated by MTT assay against cancer cell lines. Inotodiol and trametenolic acid were major triterpenoids in both of the inner and outer parts of . Inotodiol in triterpenoid fractions from the inner and outer parts of was 153.9 ± 15.4 mg/g (dry basis (db)) and 194.1 ± 11.5 mg/g, respectively. Trametenolic acid in triterpenoid fractions from the inner and outer parts of was 94.5 ± 9.15 mg/g (db) and 106.3 ± 8.23 mg/g, respectively. Triterpenoids in the outer part were significantly higher than those in the inner part. Anti-proliferative activity of the triterpenoid fraction from the outer part against AGS, MCF-7, and PC3 was also significantly higher than that of the inner part.
本研究旨在确定 内外部分提取物中的三萜类化合物组成，并评估其对癌细胞系（HT-29、AGS、MCF-7 和 PC3）的抗增殖活性。用 80%甲醇提取 内外部分 24 小时。提取物通过 Diaion HP-20 树脂进行馏分，以获得三萜类馏分。通过 HPLC 和 LC-ESI-MS 分析馏分中的三萜类化合物组成。通过 MTT 测定法评估对癌细胞系的抗增殖活性。在 内外部分中，羽扇豆醇和三萜烯酸是主要的三萜类化合物。羽扇豆醇在 内外部分的三萜类馏分中的含量分别为 153.9±15.4mg/g（干重（db））和 194.1±11.5mg/g。在 内外部分的三萜类馏分中的三萜烯酸含量分别为 94.5±9.15mg/g（db）和 106.3±8.23mg/g。外部分的三萜类化合物明显高于内部分。外部分三萜类馏分对 AGS、MCF-7 和 PC3 的抗增殖活性也明显高于内部分。
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