填充有聚（N-异丙基丙烯酰胺）人工细胞骨架的响应性粘弹性巨型脂质囊泡。

Responsive viscoelastic giant lipid vesicles filled with a poly(N-isopropylacrylamide) artificial cytoskeleton.

作者信息

Campillo Clément, Pépin-Donat Brigitte, Viallat Annie

机构信息

Adhésion et Inflammation, Inserm U600, CNRS UMR 62 12 Université Méditerranée, case 937, 163 av de Luminy, 13288 Marseille Cedex, France.

Laboratoire d'Electronique Moléculaire et Hybride, UMR 5819 SPrAM (CEA-CNRS-UJF)/DRFMC/CEA-Grenoble, 38054 Grenoble Cedex 9, France.

出版信息

Soft Matter. 2007 Oct 16;3(11):1421-1427. doi: 10.1039/b710474j.

DOI:10.1039/b710474j

PMID:32900123

Abstract

Responsive giant lipid vesicles filled with aqueous PolyNipam sol (SFV) or gel (GFV) were prepared by ultra-violet polymerisation performed in situ. Upon crossing the lower critical transition temperature of PolyNipam, SFVs and GFVs undergo a significant change of their structural and mechanical properties or a drastic volume transition, respectively. Rheometric and micropipette experiments show that both internal viscosity of SFVs and internal shear modulus of GFVs are tunable over several orders of magnitude and lie in the range observed for living cells. Moreover, the vesicle membrane is strongly bound to the internal polymer medium, making these systems interesting for mimicking the basic mechanical behaviour of passive living cells.

摘要

通过原位紫外聚合制备了填充有聚N-异丙基丙烯酰胺水溶液（SFV）或凝胶（GFV）的响应性巨型脂质囊泡。当超过聚N-异丙基丙烯酰胺的下临界转变温度时，SFV和GFV分别经历其结构和力学性能的显著变化或剧烈的体积转变。流变学和微量移液器实验表明，SFV的内部粘度和GFV的内部剪切模量均可在几个数量级内调节，且处于活细胞观察到的范围内。此外，囊泡膜与内部聚合物介质紧密结合，使得这些系统对于模拟被动活细胞的基本力学行为很有意义。