Jones C J, Bell C L, Quinton P M
Division of Biomedical Sciences, University of California, Riverside 92521-0121.
Am J Physiol. 1988 Jul;255(1 Pt 1):C102-11. doi: 10.1152/ajpcell.1988.255.1.C102.
Human eccrine sweat gland cells grown in culture were found to lose their characteristic shape, becoming flattened and organized into multilayers. The resting membrane potentials of the cultured secretory cells (-35 +/- 2 mV, n = 36) were significantly higher than those measured for cultured duct cells (-22 +/- 1 mV, n = 58, P less than or equal to 0.01). When the cholinergic agonist methacholine (10(-5) or 10(-6) M) was administered, the cultured secretory cells could be distinguished unequivocally by their atropine-sensitive hyperpolarizing response (-20 +/- 2 mV, n = 43), whereas no cultured duct cells responded. When the sodium conductance antagonist amiloride (10(-5) or 10(-6) M) was administered, 44% of cultured secretory cells responded by hyperpolarization (-8 +/- 1 mV, n = 8), whereas 87% of cultured duct cells hyperpolarized (-15 +/- 1 mV, n = 46) and by a significantly greater margin (P less than or equal to 0.01). Substitution of chloride with gluconate in the bathing medium caused membrane potential depolarization in both cultured secretory and duct cell populations, which is consistent with the presence of a chloride conductance in the plasma membrane. The beta-adrenoceptor agonist isoproterenol induced a transient hyperpolarization of 5-10 mV in three out of six cultured secretory cells tested but had no effect on cultured duct cells.
研究发现，在培养环境中生长的人外分泌汗腺细胞会失去其特有的形状，变得扁平并形成多层结构。培养的分泌细胞的静息膜电位（-35±2 mV，n = 36）显著高于培养的导管细胞（-22±1 mV，n = 58，P≤0.01）。当给予胆碱能激动剂乙酰甲胆碱（10⁻⁵或10⁻⁶ M）时，培养的分泌细胞可通过其对阿托品敏感的超极化反应（-20±2 mV，n = 43）明确区分，而培养的导管细胞无反应。当给予钠电导拮抗剂阿米洛利（10⁻⁵或10⁻⁶ M）时，44%的培养分泌细胞通过超极化反应（-8±1 mV，n = 8），而87%的培养导管细胞超极化（-15±1 mV，n = 46），且超极化幅度显著更大（P≤0.01）。在浴液培养基中用葡萄糖酸盐替代氯离子会导致培养的分泌细胞和导管细胞群体的膜电位去极化，这与质膜中存在氯电导一致。β-肾上腺素能受体激动剂异丙肾上腺素在六个测试的培养分泌细胞中有三个诱导了5 - 10 mV的瞬时超极化，但对培养的导管细胞无影响。
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