Huang Shuai, Ji Shoukun, Wang Feiran, Huang Jie, Alugongo Gibson Maswayi, Li Shengli
The State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
College of Animal Science and Technology, Hebei Agricultural University, Baoding, 071001, China.
AMB Express. 2020 Sep 17;10(1):167. doi: 10.1186/s13568-020-01106-3.
The dynamics of the community structure and composition of the dairy cow fecal bacterial communities during early lactation is unclear, therefore this study was conducted to characterize the fecal bacterial communities in dairy cows during early lactation using 16S rRNA gene sequencing. Feces were sampled from 20 healthy fresh Holstein dairy cows on day 1 (Fresh1d group) and day 14 (Fresh14d group) after calving. After calving, cows were fed the same fresh diet. The dominant phyla Firmicutes and Proteobacteria were decreased (P ≤ 0.01) with lactating progress and phyla Bacteroidetes were increased (P = 0.008) with lactating progress and dietary transition. At family level, the predominant families were Ruminococcaceae (35.23%), Lachnospiraceae (11.46%), Rikenellaceae (10.44%) and Prevotellaceae (6.89%). A total of 14 genera were different between fecal samples from Fresh1d and Fresh14d, included the predominant genera, such as Ruminococcaceae_UCG-005 (P = 0.008), Rikenellaceae_RC9_gut_group (P = 0.043) and Christensenellaceae_R-7_group (P = 0.008). All fecal bacterial communities shared members of the genera Ruminococcaceae_UCG-005, Bacteroides and Rikenellaceae_RC9_gut_group. These findings help to improve our understanding of the composition and structure of the fecal microbial community in fresh cows and may provide insight into bacterial adaptation time and dietary in lactating cows.
奶牛泌乳早期粪便细菌群落的结构和组成动态尚不清楚，因此本研究采用16S rRNA基因测序对奶牛泌乳早期的粪便细菌群落进行特征分析。在产犊后第1天（产后1天组）和第14天（产后14天组）从20头健康的初产荷斯坦奶牛采集粪便。产犊后，奶牛饲喂相同的新鲜日粮。优势菌门厚壁菌门和变形菌门随着泌乳进程而减少（P≤0.01），拟杆菌门随着泌乳进程和日粮转变而增加（P = 0.008）。在科水平上，优势科为瘤胃球菌科（35.23%）、毛螺菌科（11.46%）、理研菌科（10.44%）和普雷沃氏菌科（6.89%）。产后1天和产后14天的粪便样本共有14个属存在差异，包括优势属，如瘤胃球菌科_UCG-005（P = 0.008）理研菌科_RC9肠道菌群（P = 0.043）和克里斯滕森菌科_R-7菌群（P = 0.008）。所有粪便细菌群落都有瘤胃球菌科_UCG-005、拟杆菌属和理研菌科_RC9肠道菌群的成员。这些发现有助于提高我们对初产奶牛粪便微生物群落组成和结构的理解，并可能为泌乳奶牛的细菌适应时间和日粮提供见解。
Zotero 插件
