State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, PR China.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, PR China.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5747-5752. doi: 10.1099/ijsem.0.004472.
A Gram-stain-negative, aerobic, polarly flagellated, straight or curved rod-shaped bacterium, designated strain M1K-6, was isolated from deep seawater samples collected from the Mariana Trench. The strain grew at -4 to 37 °C (optimum, 25-30 °C), at pH 5.5-10.0 (optimum, pH 7.0) and with 0.5-14.0 % (w/v) NaCl (optimum, 2.0 %). It did not reduce nitrate to nitrite nor hydrolyse gelatin or starch. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M1K-6 was affiliated with the genus , sharing 93.1-97.0 % sequence similarity with the type strains of recognized species. The major cellular fatty acids were summed feature 3 (C 6/C 7), summed feature 8 (C 7/C 6), C, C 3-OH and C. The predominant respiratory quinone was ubiquinone-8. Polar lipids of strain M1K-6 included phosphatidylethanolamine, phosphatidylglycerol and two unidentified lipids. The genomic G+C content of strain M1K-6 was 46.0 mol%. Based on data from the present polyphasic study, strain M1K-6 was considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is M1K-6 (=KCTC 72501=MCCC 1K03890).
一株革兰氏阴性、需氧、极生鞭毛、直或弯曲的杆状细菌，被命名为 M1K-6 菌株，从马里亚纳海沟的深海水样中分离得到。该菌株在-4 到 37°C（最适温度为 25-30°C）、pH5.5-10.0（最适 pH7.0）和 0.5-14.0%（w/v）NaCl（最适 2.0%）的条件下生长。它不能将硝酸盐还原为亚硝酸盐，也不能水解明胶或淀粉。基于 16S rRNA 基因序列的系统发育分析表明，M1K-6 菌株与属密切相关，与已鉴定的种的模式菌株的序列相似度为 93.1-97.0%。主要细胞脂肪酸为 C6/C7 总和特征 3、C7/C6 总和特征 8、C、C3-OH 和 C。主要呼吸醌为泛醌-8。M1K-6 菌株的极性脂包括磷脂酰乙醇胺、磷脂酰甘油和两种未鉴定的脂类。M1K-6 菌株的基因组 G+C 含量为 46.0mol%。基于多相研究的现有数据，M1K-6 菌株被认为代表属内的一个新种，建议将其命名为 sp. nov.。模式菌株为 M1K-6（=KCTC 72501=MCCC 1K03890）。
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