Spondin-2 的过表达与局限性透明细胞肾细胞癌患者的无复发生存相关。
Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma.
机构信息
Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001 Anhui, China.
Key Laboratory of Noncoding RNA Transformation Research of Anhui Higher Education Institution (Wannan Medical College), Wuhu, 241002 Anhui, China.
出版信息
Dis Markers. 2020 Sep 3;2020:5074239. doi: 10.1155/2020/5074239. eCollection 2020.
BACKGROUND
The spondin-2 (SPON2) gene is overexpressed in multiple malignant tumors and may promote tumor aggressiveness. However, its expression profile and functional roles in clear cell renal cell carcinoma (ccRCC) are still unclear.
METHODS
SPON2 expression in ccRCC was evaluated using expression data from TCGA and GEO databases, then confirmed by local patient population (94 patients). The clinical significance of SPON2 expression was evaluated. Downregulation of SPON2 was performed using small-interfering RNA (siRNA). The effects of SPON2 silencing on cell proliferation, apoptosis, invasion, and migration were investigated.
RESULTS
SPON2 was overexpressed in the majority of the ccRCC at both mRNA and protein levels. SPON2 expression was significantly correlated with stage, grade, and recurrence (all < 0.05) in patients with localized ccRCC. The receiver operating characteristic (ROC) curve showed that SPON2 expression could serve as a predictor of recurrence. SPON2 expression was significantly associated with recurrence-free survival (RFS) in patients with localized ccRCC. Knocking down SPON2 resulted in suppressed cell invasion and migration .
CONCLUSION
SPON2 expression might function as a prognostic biomarker in patients with localized ccRCC.
背景
卷曲相关蛋白 2（SPON2）基因在多种恶性肿瘤中过度表达，可能促进肿瘤侵袭性。然而，其在透明细胞肾细胞癌（ccRCC）中的表达谱和功能作用尚不清楚。
方法
使用 TCGA 和 GEO 数据库中的表达数据评估 SPON2 在 ccRCC 中的表达，然后通过本地患者群体（94 例）进行验证。评估 SPON2 表达的临床意义。使用小干扰 RNA（siRNA）下调 SPON2 的表达。研究 SPON2 沉默对细胞增殖、凋亡、侵袭和迁移的影响。
结果
SPON2 在大多数 ccRCC 中的 mRNA 和蛋白水平均过度表达。SPON2 表达与局限性 ccRCC 患者的分期、分级和复发显著相关（均<0.05）。受试者工作特征（ROC）曲线表明，SPON2 表达可作为复发的预测因子。SPON2 表达与局限性 ccRCC 患者的无复发生存（RFS）显著相关。敲低 SPON2 导致细胞侵袭和迁移受到抑制。
结论
SPON2 表达可能是局限性 ccRCC 患者的预后生物标志物。
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