Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen 518036, China; Peking University Shenzhen Hospital Clinical College, Anhui Medical University, Hefei 230032, China; Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, China.
Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen 518036, China; Shenzhen Baoan Maternal and Child Health Hospital, Shenzhen 518000, China; Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, China.
Genomics. 2020 Nov;112(6):5275-5281. doi: 10.1016/j.ygeno.2020.09.035. Epub 2020 Sep 18.
Oral squamous cell carcinoma (OSCC) is a common and rampant malignancy of the head and neck. However, its pathogenesis remains unclear. In this study, we have investigated the effects of circular RNA hsa_circ_009755 on proliferation, migration, invasion, and apoptosis in OSCC cells in vitro.
Eight pairs of OSCC and normal adjacent tissues were selected to detect the differential expression of circRNAs by high-throughput sequencing. circRNA hsa_circ_009755 expression was determined by quantitative real-time polymerase chain reaction in OSCC tissues and cell lines. CCK-8, wound healing, Transwell, and flow cytometry assays were used to determine OSCC cell proliferation, migration, invasion, and apoptosis, respectively.
The expression of hsa_circ_009755 was low in OSCC tissues and three OSCC cell lines. Silencing hsa_circ_009755 significantly enhanced the proliferation, migration, and invasion and suppressed the apoptosis of OSCC cells.
Therefore, hsa_circ_009755 may be important in the tumorigenesis of OSCC.
口腔鳞状细胞癌（OSCC）是一种常见且肆虐的头颈部恶性肿瘤。然而，其发病机制尚不清楚。在本研究中，我们研究了环状 RNA hsa_circ_009755 对体外 OSCC 细胞增殖、迁移、侵袭和凋亡的影响。
选择 8 对 OSCC 组织和正常相邻组织，通过高通量测序检测 circRNAs 的差异表达。通过定量实时聚合酶链反应检测 OSCC 组织和细胞系中 circRNA hsa_circ_009755 的表达。CCK-8、划痕愈合、Transwell 和流式细胞术分别用于测定 OSCC 细胞的增殖、迁移、侵袭和凋亡。
hsa_circ_009755 在 OSCC 组织和三种 OSCC 细胞系中的表达较低。沉默 hsa_circ_009755 可显著增强 OSCC 细胞的增殖、迁移和侵袭，并抑制其凋亡。
因此，hsa_circ_009755 可能在 OSCC 的肿瘤发生中起重要作用。
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