State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, 430062, Wuhan, P. R. China.
State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, 200237, Shanghai, P. R. China.
Nat Commun. 2020 Oct 7;11(1):5035. doi: 10.1038/s41467-020-18833-7.
Aliphatic α,ω-dicarboxylic acids (DCAs) are a class of useful chemicals that are currently produced by energy-intensive, multistage chemical oxidations that are hazardous to the environment. Therefore, the development of environmentally friendly, safe, neutral routes to DCAs is important. We report an in vivo artificially designed biocatalytic cascade process for biotransformation of cycloalkanes to DCAs. To reduce protein expression burden and redox constraints caused by multi-enzyme expression in a single microbe, the biocatalytic pathway is divided into three basic Escherichia coli cell modules. The modules possess either redox-neutral or redox-regeneration systems and are combined to form E. coli consortia for use in biotransformations. The designed consortia of E. coli containing the modules efficiently convert cycloalkanes or cycloalkanols to DCAs without addition of exogenous coenzymes. Thus, this developed biocatalytic process provides a promising alternative to the current industrial process for manufacturing DCAs.
脂肪族 α,ω-二羧酸（DCAs）是一类有用的化学品，目前通过能源密集型、多阶段的化学氧化法生产，对环境有危害。因此，开发环境友好、安全、中性的 DCA 合成路线非常重要。我们报告了一种体内人工设计的生物催化级联过程，用于将环烷烃生物转化为 DCAs。为了降低因在单个微生物中表达多种酶而导致的蛋白表达负担和氧化还原限制，生物催化途径被分为三个基本的大肠杆菌细胞模块。这些模块具有氧化还原中性或氧化还原再生系统，并被组合成用于生物转化的大肠杆菌联合体。含有这些模块的设计好的大肠杆菌联合体可以有效地将环烷烃或环烷醇转化为 DCAs，而无需添加外源辅酶。因此，这种开发的生物催化过程为制造 DCA 的现有工业过程提供了一种很有前途的替代方法。
