sp. nov. isolated from culture of microalga sp.

A novel Gram-stain-negative, aerobic, non-spore-forming, non-motile, and rod-shaped bacterium, strain ETT8 was isolated from a chemostat culture of microalga sp. YC001. Optimal growth was with 0-2% NaCl and at 25-37 °C on R2A medium. Phylogenetic analysis based on the 16S rRNA gene and genome sequence showed that strain ETT8 belongs to the genus , with the close neighbours being DRYC-M-16 (98.1 %), DJC (97.6 %), SY72 (96.9 %), K13M18 (96.8 %), and RCRI19 (96.5 %). The genomic comparison of strain ETT8 with type species in the genus was analysed using the genome-to-genome distance calculator (GGDC), average nucleotide identity (ANI), and average amino acid identity (AAI) (values indicated ≤17.7, ≤75.4 and ≤71.9 %, respectively). The genomic DNA G+C content of strain ETT8 was 64.4 %, plus C c and C-iso were the major fatty acids and Q-10 the major respiratory quinone. Strain ETT8 contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine aminolipid, and four unidentified lipids as the major polar lipids. Based on the chemotaxonomic, genotypic, and phenotype results, strain ETT8 was recognized as a novel species of the genus for which the name sp. nov. is proposed. The type strain is ETT8 (=KCTC 72206=JCM 31893=MCC 4339).