Agricultural Big-Data Research Center and College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
Beijing Key Laboratory of Grape Science and Enology, and CAS Key Laboratory of Plant Resources, Institute of Botany, Innovation Academy for Seed Design, the Chinese Academy of Science, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Gene. 2021 Feb 15;769:145226. doi: 10.1016/j.gene.2020.145226. Epub 2020 Oct 12.
Abscisic acid (ABA) plays important roles in plant development and tolerance to abiotic stresses. Limited information is available regarding ABA signaling pathway genes in grape. In this study, 9 VvPYR/PYLs, 85 VvPP2Cs, 7 VvABIs, 7 VvSnRK2s, and 8 VvABFs were identified in the grape genome. Duplication analysis indicated that whole genome duplication might contribute to the expansion of these gene families. The comprehensive transcriptome analysis in various organs/tissues implied that most of these genes were tissue-specific, and few were environment-specific genes. Exogenous ABA treatment reduced the grape maturation period. VvPP2C59, VvPP2C60, VvPP2C66, and VvABF8 were all involved in tolerance to cold, heat, and drought stresses, revealing their crucial roles in regulating environmental stress responses. This work provides detailed information of ABA signaling pathway genes and new insights regarding their expression patterns during grape development and abiotic stress treatment.
脱落酸(ABA)在植物发育和非生物胁迫耐受中发挥重要作用。关于葡萄中的 ABA 信号通路基因,信息有限。在本研究中,在葡萄基因组中鉴定出 9 个 VvPYR/PYLs、85 个 VvPP2Cs、7 个 VvABIs、7 个 VvSnRK2s 和 8 个 VvABFs。复制分析表明,全基因组复制可能有助于这些基因家族的扩张。在各种器官/组织中的综合转录组分析表明,这些基因大多数具有组织特异性,少数是环境特异性基因。外源 ABA 处理可缩短葡萄成熟期。VvPP2C59、VvPP2C60、VvPP2C66 和 VvABF8 均参与冷、热和干旱胁迫耐受,表明它们在调节环境胁迫反应中起着关键作用。这项工作提供了 ABA 信号通路基因的详细信息,并深入了解了它们在葡萄发育和非生物胁迫处理过程中的表达模式。