Department of Bioengineering, University of California, Berkeley, CA, 94720, USA; UC Berkeley-UCSF Graduate Program in Bioengineering, USA.
Department of Bioengineering, University of California, Berkeley, CA, 94720, USA; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, 94720, USA; UC Berkeley-UCSF Graduate Program in Bioengineering, USA.
Curr Opin Cell Biol. 2021 Feb;68:64-71. doi: 10.1016/j.ceb.2020.08.016. Epub 2020 Oct 16.
Engineered culture substrates have proven invaluable for investigating the role of cell and extracellular matrix geometry in governing cell behavior. While the mechanisms relating geometry to phenotype are complex, it is clear that the actin cytoskeleton plays a key role in integrating geometric inputs and transducing these cues into intracellular signals that drive downstream biology. Here, we review recent progress in elucidating the role of the cell and matrix geometry in regulating actin cytoskeletal architecture and mechanics. We address new developments in traditional two-dimensional culture paradigms and discuss efforts to extend these advances to three-dimensional systems, ranging from nanotextured surfaces to microtopographical systems (e.g. channels) to fully three-dimensional matrices.
工程化的细胞培养基质在研究细胞和细胞外基质的几何形状在调控细胞行为中的作用方面已经被证明是非常有价值的。尽管将几何形状与表型联系起来的机制很复杂，但很明显，肌动蛋白细胞骨架在整合几何输入并将这些线索转化为驱动下游生物学的细胞内信号方面起着关键作用。在这里，我们回顾了阐明细胞和基质几何形状在调节肌动蛋白细胞骨架结构和力学方面的最新进展。我们讨论了传统二维培养范式中的新进展，并讨论了将这些进展扩展到三维系统的努力，从纳米纹理表面到微地形系统（例如通道）再到完全的三维基质。
