Hakimian Fatemeh, Ghourchian Hedayatollah
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, P.O. Box 13145-1384, Iran; Medical Nanotechnology & Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, P.O. Box 13145-1384, Iran.
Anal Chim Acta. 2020 Nov 1;1136:1-8. doi: 10.1016/j.aca.2020.08.039. Epub 2020 Aug 28.
Due to the stability of microRNAs (miRNAs) in serum and other body fluids, they are known as promising cancer biomarkers. Recent studies have indicated higher expression of miRNA-155 (miR-155) in patients with breast cancer compared to healthy people. In the present report, a rapid and sensitive electrochemical biosensor has been developed for detection of miR-155 as a breast cancer risk factor. At first, a thiolated probe was immobilized on the gold electrode surface. Then, the target (miR-155) was exposed to the probe. In the next step, the positively charged polyethyleneimine-silver nanoparticles as electroactive labels were absorbed onto the negatively charged probe-target hybrid. In the third step, the anodic peak current which was produced due to the oxidation of silver nanoparticles was recorded as the electrochemical signal. The designed biosensor provided an ultrasensitive method for the detection of miR-155 with the detection limit of 20 zmol and a wide linear range from 2 × 10 to 2 × 10 mol. Moreover, the biosensor was able to detect miR-155 in real serum samples with satisfactory results.
由于微小RNA（miRNA）在血清和其他体液中具有稳定性，它们被认为是很有前景的癌症生物标志物。最近的研究表明，与健康人相比，乳腺癌患者中miRNA - 155（miR - 155）的表达更高。在本报告中，已开发出一种快速灵敏的电化学生物传感器，用于检测作为乳腺癌风险因素的miR - 155。首先，将硫醇化探针固定在金电极表面。然后，使靶标（miR - 155）与探针接触。下一步，将带正电荷的聚乙烯亚胺 - 银纳米颗粒作为电活性标记物吸附到带负电荷的探针 - 靶标杂交体上。第三步，将由于银纳米颗粒氧化产生的阳极峰电流记录为电化学信号。所设计的生物传感器提供了一种超灵敏的检测miR - 155的方法，检测限为20 zeptomole，线性范围宽，从2×10到2×10摩尔。此外，该生物传感器能够检测实际血清样品中的miR - 155，结果令人满意。
